167,564 results

1. pAC1-V16-hGDNF

   Plasmid

   #122035

   Purpose

   AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

   Depositor

   Liliane Tenenbaum

   Article

   Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

   Insert

   hGDNF (GDNF Human)
   

   Use

   AAV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTight-hMYT1L-N174

   Plasmid

   #31877

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   myelin transcription factor 1-like (MYT1L Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167937

   Purpose

   Inducible CRISPRa expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   dxCas9(3.7)-VPR
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-HA-AR-NTD

   Plasmid

   #171235

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human SERCA2b (pcDNA3.1+)

   Plasmid

   #75188

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   David MacLennan, Jonathan Lytton

   Article

   Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

   Insert

   ATP2A2 (ATP2A2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTarget_CAST

   Plasmid

   #127926

   Purpose

   Target plasmid containing PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   PSP1 target (GGTT PAM)
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER-RpHLuorin2

   Plasmid

   #171717

   Purpose

   Luminal ER expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 targeted to ER
   

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-hygro-STING

   Plasmid

   #102598

   Purpose

   Express STING for IFN-beta luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_TGFBR1_WT

    Plasmid

    #82237

    Purpose

    Gateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TGFBR1 (TGFB1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL50009

    Plasmid

    #50309

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. plRL19

    Plasmid

    #163634

    Purpose

    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Insert

    L5 Integrase
    

    Expression

    Bacterial

    Promoter

    Mycobacterial Optimized Promoter (MOP) and Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Ryota Uehara, Gohta Goshima

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 MYC-Sumo3 WT

    Plasmid

    #48964

    Purpose

    Expresses MYC-Sumo3 Wild Type in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO3 (SUMO3 Human)
    

    Tags

    MYC (EQKLISEED)

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBBR15.2-2H12.D11opt

    Plasmid

    #221185

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2.1
    

    Expression

    Bacterial

    Promoter

    Constitutive

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF112

    Plasmid

    #135920

    Purpose

    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Type

    Empty backbone

    Use

    Luciferase; Transposon

    Expression

    Mammalian

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

    Plasmid

    #133028

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Golgin84 (GOLGA5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-673

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP1-10-miniCMV-GFP11×11-GFP-tDeg

    Plasmid

    #185404

    Purpose

    Expresses GFP1-10 and GFP11×11-GFP-tDeg

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    GFP1-10
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

    Plasmid

    #162230

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMRE-Tn7-163

    Plasmid

    #118575

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBE48

    Plasmid

    #174656

    Purpose

    CAPTURE method BAC origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7-EGFP-C1-HsDCP2

    Plasmid

    #25031

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DCP2 (DCP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223_SRC_WT

    Plasmid

    #82165

    Purpose

    Gateway Donor vector containing SRC , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SRC (SRC Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDendra2-H2b

    Plasmid

    #157817

    Purpose

    Fluorescent reporter for histone H2B tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    Histone H2B (H2bc3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-loxpSTOPloxp-lyn-NeonGreen

    Plasmid

    #175257

    Purpose

    membrane target protein labelling

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    Lyn membrane targeting motif
    

    Use

    Cre/Lox

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pCAG

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p6599 MSCV-IP N-HAonly FOSL1

    Plasmid

    #34897

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. VAMP3-mCherry

    Plasmid

    #92423

    Purpose

    Expression of VAMP3 C-terminally conjugated to mCherry.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    VAMP3 (Vamp3 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-EF1-IRES-BSD

    Plasmid

    #209751

    Purpose

    Lentiviral transfer plasmid to express the blasticidin resistance gene

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV.CAG.iAChSnFR (AAV9)

    Viral Prep

    #137955-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.CAG.iAChSnFR (#137955). In addition to the viral particles, you will also receive purified pAAV.CAG.iAChSnFR plasmid DNA. CAG-driven iAChSnFR acetylcholine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Promoter

    CAG

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

32. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

    Plasmid

    #202689

    Purpose

    CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Inserts

    CopGFP
    mCherry
    

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCherry-Rab5a-7

    Plasmid

    #55126

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pICH47742

    Plasmid

    #48001

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR223_H2AFX_WT

    Plasmid

    #81937

    Purpose

    Gateway Donor vector containing H2AFX, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    H2AFX (H2AX Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBsaBM3

    Plasmid

    #85102

    Purpose

    P450 BM3 variant (A74G/C773S/C810S)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    P450 BM3
    

    Use

    Cloning vector

    Mutation

    A74G/C773S/C810S

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAS1-Prig-3::tagBFP

    Plasmid

    #165598

    Purpose

    rig-3 promoter derive BFP expression in AVA head neurons.

    Depositor

    Andrew Leifer

    Article

    Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

    Insert

    Prig-3, tagBFP
    

    Expression

    Worm

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PX551

    Plasmid

    #60957

    Purpose

    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSKA413

    Plasmid

    #80381

    Purpose

    Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    ccaS (complement)
    ccaR (complement)
    gfpmut3
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    FLAG tag

    Expression

    Bacterial

    Promoter

    ccaR and cpcG2Δ59

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Lenti-CMV-MLH1dn-IRES-puroR

    Plasmid

    #207354

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    MutL homolog 1 (MLH1 Human)
    

    Use

    Lentiviral

    Mutation

    Δ754-756 dominant negative mutant

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJC005.em

    Plasmid

    #182738

    Purpose

    CRISPR-Cas12a genome editing of E. faecium

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TFORF0276

    Plasmid

    #142386

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Flag-neo-PP1-Beta

    Plasmid

    #171269

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunit

    Depositor

    Steven Balk

    Insert

    3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Expression

    Worm

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. YFP-NOP58-C1

    Plasmid

    #134537

    Purpose

    Expresses YFP-NOP58 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    NOP58 (NOP58 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. TFORF1898

    Plasmid

    #144116

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV2-CAG-3xNLS-mScarlet

    Plasmid

    #191098

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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