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Showing: 6571 - 6600 of 160648 results

  1. pFUGW-FIRE-pHLy

    Plasmid
    #170774
    Purpose
    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    hUbC
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170774-plasmid-map-sequence-id-339008

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  10. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid
    #123350
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-Cameleon
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123350-plasmid-map-sequence-id-307103

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  11. pSU315

    Plasmid
    #169697
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169697-plasmid-map-sequence-id-334518

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  14. pAL-rfp_T

    Plasmid
    #74075
    Purpose
    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA rfp (T)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74075-plasmid-map-sequence-id-141966

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  19. pAAV-Syn-Flex-TREx2-tTA

    Plasmid
    #191210
    Purpose
    Cre dependent TRE-tTA expression under the control of Syn promoter
    Depositor
    Janelia Viral Tools
    Insert
    tTA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    191210-plasmid-map-sequence-id-374532

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  22. pAL-pck_T

    Plasmid
    #74068
    Purpose
    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pck (T)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. pD649-HAsp-CEACAM4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156496
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CEACAM4 (CEACAM4 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    156496-plasmid-map-sequence-id-368821

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  27. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123329
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-AKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123329-plasmid-map-sequence-id-313436

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Showing: 6571 - 6600 of 160648 results