164,909 results

1. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HRE-oUnaG

   Plasmid

   #201712

   Purpose

   Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSMALB-ATF4.5

   Plasmid

   #155032

   Purpose

   Fluorescent reporter for ATF4 translation

   Depositor

   John Dick, Peter van Galen

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGFP

   Plasmid

   #32748

   Depositor

   Jeanne Hardy

   Article

   Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

   Insert

   GFP
   

   Use

   Tags

   DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

   Expression

   Mammalian

   Mutation

   S65T

   Promoter

   CMV

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV_BiVIPe4_GCaMP6f

   Plasmid

   #213859

   Purpose

   AAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-nEF-Con/Fon-NpHR3.3-EYFP (AAV8)

   Viral Prep

   #137152-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-NpHR3.3-EYFP (#137152). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of NpHR3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre and Flp-dependent)

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189925

   Purpose

   AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SauriABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SauriCas9 D15A

   Promoter

   EFS

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLBS-mScarlet

   Plasmid

   #129337

   Purpose

   cytosol marker, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   mScarlet (Addgene 85042)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p006-GFP-strong

    Plasmid

    #108313

    Purpose

    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4026

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PermaPhos Kit

    Plasmid Kit

    #1000000226

    Purpose

    This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

    Depositor

    Ryan Mehl

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

13. wt HA-dynamin 1 pcDNA3.1

    Plasmid

    #34682

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. hFLT1

    Plasmid

    #83435

    Purpose

    human VEGFR1 with HA and FLAG tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

    Insert

    human VEGFR1 (FLT1 Human)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    HA-tag was inserted 30 AA downstream of the FLT1 …

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF0373

    Plasmid

    #141594

    Purpose

    Lentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFYA (NFYA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-NSP1SA11

    Plasmid

    #89168

    Purpose

    Express Rotavirus SA11 NSP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mouse genome-wide lentiviral CRISPR gRNA library version 2

    Pooled Library

    #67988

    Purpose

    Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Species

    Homo sapiens, Mus musculus, and Synthetic

    Use

    CRISPR and Lentiviral

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-LoxP-mKate-LoxP-EGFP

    Plasmid

    #167940

    Purpose

    CMV promoter-LoxP-mKate-LoxP-EGFP

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    LoxP-mKate-LoxP-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Halo-MTS

    Plasmid

    #124315

    Purpose

    Expresses mitochondria specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    Mito specific seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRXIP VPS16-GFP

    Plasmid

    #67023

    Purpose

    Human VPS16 ORF was inserted into pMRXIP GFP-N vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

21. MSCV-N BNLF2a

    Plasmid

    #37941

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BNLF2a (BNLF2a H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEZYflag

    Plasmid

    #18700

    Purpose

    pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-MTS-jAspSnFR3-mRuby3

    Plasmid

    #203492

    Purpose

    Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3-mRuby3
    

    Use

    Lentiviral

    Tags

    His-tag, Mitochondrial localization signal, and m…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJL-TRBO-G

    Plasmid

    #80083

    Purpose

    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLX302

    Plasmid

    #25896

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Human DNA Binding Domain-Focused CRISPR Knockout Library

    Pooled Library

    #123334

    Purpose

    Expresses gRNA targeting the DNA binding domain of human transcription factors.

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

28. pFW6000

    Plasmid

    #224215

    Purpose

    Bacteroides genomic editing tool; aTc inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHIS3p:CloverGFP-Tub1+3'UTR::URA3

    Plasmid

    #50636

    Depositor

    Wei-Lih Lee

    Article

    Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

    Insert

    CloverGFP-Tub1
    

    Use

    Yeast expression

    Tags

    CloverGFP

    Expression

    Mutation

    Promoter

    HIS3p

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF3081

    Plasmid

    #144557

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

    Plasmid

    #85428

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-12
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF1977

    Plasmid

    #144120

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX1 (TBX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid

    #159904

    Purpose

    Mutagenesis of Kcnn3

    Depositor

    Larry Zweifel

    Insert

    Kcnn3 (Kcnn3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV_S5E2_Chrimson_tdTomato

    Plasmid

    #219432

    Purpose

    AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

    Depositor

    Gordon Fishell

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    Chrimson-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

    Plasmid

    #134859

    Purpose

    Expresses EGFP-tagged ERGIC marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    ERGIC53 (Lman1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HA-Rab11-WT

    Plasmid

    #101047

    Purpose

    Expresses HA-tagged human Rab11-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-C-terminal GST

    Plasmid

    #128025

    Purpose

    Mammalian expression vector with C terminal GST tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-PV-NLS-GFP

    Plasmid

    #17300

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-GFP-NLS (Pvalb Rat)
    

    Use

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

    Plasmid

    #63166

    Purpose

    GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-HA"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62904

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAGGS-ChR2-Venus

    Plasmid

    #15753

    Purpose

    Expresses ChR2-Venus for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

    Insert

    Channelrhodopsin2-Venus
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pORANGE GFP-Grin1 KI

    Plasmid

    #131485

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AAV-hRedOpsin-TGFB1

    Plasmid

    #164427

    Purpose

    AAV plasmid expressing TGFB1 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

    Insert

    Tgfb1 (Tgfb1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human red opsin

    Available since

    Nov. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. His-Tev-UbE1

    Plasmid

    #186804

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE1 (UBA1 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. Mito-mEosEM

    Plasmid

    #132706

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    mitochondria targeting sequence
    

    Use

    Tags

    mEosEM

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. GFP-hAgo2

    Plasmid

    #11590

    Depositor

    Edward Chan

    Article

    Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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48. DsRed-Rab5 WT

    Plasmid

    #13050

    Depositor

    Richard Pagano

    Article

    Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

    Insert

    RAB5A (Canis familiaris) (RAB5A Canis familiaris)
    

    Use

    Tags

    DsRed2 and HA

    Expression

    Mammalian

    Mutation

    asparagine 3 changed to serine (neutral); serine …

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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49. pTRIP-CMV-mCherry-53BP1

    Plasmid

    #127658

    Purpose

    Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. Mito-SBFP2

    Plasmid

    #187964

    Purpose

    Mammalian cell expression of a fluorescent SBFP2-tagged mitochondrial marker

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    SBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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