164,868 results

1. pHAGE-PIK3CA-H1047R

   Plasmid

   #116500

   Purpose

   Lentiviral expression of PIK3CA H1047R

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   H1047R

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe EGFR(T790M)

   Plasmid

   #32070

   Depositor

   Matthew Meyerson

   Article

   Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   T790M

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Fab_8D3_2_H-H6

   Plasmid

   #176075

   Purpose

   Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   His6 tagged heavy chain of Fab_8D3_2
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Fab_8D3_2_L

   Plasmid

   #176076

   Purpose

   Express light chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   Light chain of Fab_8D3_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSRE SSG

   Plasmid

   #127692

   Purpose

   Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Structural Genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid

    #185919

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pATetO 6XHis-Phusion

    Plasmid

    #206210

    Purpose

    Inducible expression of DNA polymerase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    Phusion DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZP06B

    Plasmid

    #232321

    Purpose

    Make sacB a counterselection marker to replace galK

    Depositor

    Chenggang Wu

    Insert

    PrpsJ-sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-NES-jRCaMP1b

    Plasmid

    #63136

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1b
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C9orf72

    Plasmid

    #83437

    Purpose

    Expresses GFP-C9orf72 in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    C9orf72 (C9orf72 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Puro_siKD

    Plasmid

    #86695

    Purpose

    Tetracycline inducible expression of shRNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 TO

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-hsyn-ASAP5-WPRE

    Plasmid

    #225709

    Purpose

    Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

    Pooled Library

    #84003

    Purpose

    Mouse CRISPRa Pooled Library targeting membrane proteins

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

24. pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

    Plasmid

    #60660

    Purpose

    Flp-dependent expression of mNaChBacMut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX-mNaChBacMut-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E191K

    Promoter

    EF1α

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Paul Blainey, Stephen Quake

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Use

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAdEasy-1

    Plasmid

    #16400

    Purpose

    Adenoviral vector for use in AdEasy System.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGL3 RARE-RFP

    Plasmid

    #183054

    Purpose

    Fluorescent reporter for Retinoic Acid activity

    Depositor

    Chaya Kalcheim

    Article

    Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    RARE, SV40

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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