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pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

Plasmid

#141198

Purpose

Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids and amino acids 6…

Promoter

CMV

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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hα1Ga-pDsRed

Plasmid

#45811

Depositor

Edward Perez-Reyes

Insert

hα1Ga (CACNA1G Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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SIV_Gag_Pol

Plasmid

#236243

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Gag and Pol

Use

Lentiviral

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-FBXO22

Plasmid

#232271

Purpose

Expression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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PB-empty-DSE-TetO-8kb

Plasmid

#213022

Purpose

piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetO

Use

Mouse Targeting; Piggybase integration (transposo…

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-V5/HIS

Plasmid

#205617

Purpose

Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR2 receptor tyrosine kinase (KDR Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-PML-IV

Plasmid

#62804

Purpose

expresses the isoform IV of PML (promyelocytic leukemia protein)

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only
pSMPP-mCherry-hTRIM21

Plasmid

#104972

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Lentiviral

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-hHMGCR1

Plasmid

#86085

Purpose

expresses the human HMG-CoA reductase isoform 1

Depositor

Anne Galy

Insert

human HMG-CoA Reductase isoform 1 (HMGCR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only
pMSCV-miR30-shJAG1 #1

Plasmid

#171196

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #1 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #1

Use

Retroviral

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB-cLUC

Plasmid

#174051

Purpose

Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

Depositor

Alberto Macho

Article

Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

Type

Empty backbone

Use

Luciferase

Expression

Plant

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SRE reporter vector_559

Plasmid

#82686

Purpose

Cloning vector containing serum response element (SRE) followed by an AscI restriction site

Depositor

Harald Janovjak

Article

Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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modRNAc1-VP64dCas9VP64-2A-MPH

Plasmid

#216388

Purpose

this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

VP64dCas9VP64-2A-MPH

Use

CRISPR

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nHA Spinophilin

Plasmid

#87122

Purpose

mammalian expression of Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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rAAV2/cMG

Plasmid

#184539

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A589D; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)

Plasmid

#228881

Purpose

PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

Building blocks of GEMINI for intracellular assembly

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRK24

Bacterial Strain

#51950

Purpose

broad-host range natural conjugative plasmid

Depositor

Farren Isaacs

Article

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

Bacterial Resistance

Tetracycline

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only
AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

Plasmid

#230360

Purpose

AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV2)

Viral Prep

#87306-AAV2

Purpose

Ready-to-use AAV2 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Promoter

EF1a

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only
pInducer-YAP1-S127A

Plasmid

#213584

Purpose

Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max_P2A_GFP

Plasmid

#112100

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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PAmCherry-N1-FRB-Tubbyc

Plasmid

#202678

Purpose

Photoactivatable and rapamycin recruitable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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ZJOM18

Plasmid

#133631

Purpose

Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-NAB2-GFP (NAB2 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.3b (AAV5)

Viral Prep

#125560-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Promoter

CAG

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only