168,004 results

1. pCR1265

   Plasmid

   #111094

   Purpose

   lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC475 - pAAV c-fos iRFP

   Plasmid

   #47906

   Purpose

   An AAV vector that expresses iRFP under the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   c-fos

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGT-S1

   Plasmid

   #122582

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CLYBL_Bxb1-GA_LP_Dual_TC

   Plasmid

   #229776

   Purpose

   CLYBL targeting construct with the Bxb1-GA landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   FRT-mScarlet-attP(GA)-PuroR-F3
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pGK

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Expression

   Mammalian

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. shp53 pLKO.1 puro

   Plasmid

   #19119

   Purpose

   3rd gen lentiviral vector for knocking down p53 gene expression

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against tumor protein p53 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. Salsa-BC1-P1A1

   Plasmid

   #202842

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC1
   

   Expression

   Mammalian

   Promoter

   Tet Response Element (TRE)

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 flag p53

   Plasmid

   #10838

   Depositor

   Thomas Roberts

   Article

   Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

   Insert

   p53 (TP53 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV IRES Luciferase

   Plasmid

   #18760

   Depositor

   Scott Lowe

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE HA Chk2 WT

    Plasmid

    #41901

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    CHK2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2-sgCTRL

    Plasmid

    #209750

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    Cas9
    

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3254

    Plasmid

    #144730

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG2-EGFP-C-IFT144

    Plasmid

    #218740

    Purpose

    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT144 (WDR19 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Halo-TOMM20-N-10

    Plasmid

    #123284

    Purpose

    Localization: Outer Mitochondrial Membrane

    Depositor

    Kevin McGowan

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL Blast GFP

    Plasmid

    #12269

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pThermoCas9_ctrl

    Plasmid

    #100981

    Purpose

    Expresses ThermoCas9 and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-USP39

    Plasmid

    #22581

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP39 (USP39 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pRU42

    Plasmid

    #167664

    Purpose

    ENTRY vector for gRNA2 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBAD33-mf-lon

    Plasmid

    #21867

    Depositor

    Robert Sauer

    Article

    Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    Lon (Mfl404 Mesoplasma florum)
    

    Expression

    Bacterial

    Mutation

    all TGA codons changed to TGG codons

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. p426MET25_sfpHluorin (MRV55)

    Plasmid

    #115697

    Purpose

    superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    sfpHluorin
    

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid

    #203350

    Purpose

    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Molly Stevens, Christopher Grigsby

    Insert

    Nano Luciferase
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Promoter

    T7

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. T7-VEE-OKS-iG

    Plasmid

    #58974

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV BiP-Myc-KDEL-wt

    Plasmid

    #27164

    Purpose

    Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLIX403-EYFP-ccdB-Hygro

    Plasmid

    #158555

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTW335c3

    Plasmid

    #237322

    Purpose

    expresses A. thaliana RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Inserts

    A. thaliana RbcL (rbcL Mustard Weed)
    A. thaliana RbcS-6xHis
    

    Tags

    6xHis and None

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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31. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-Casp8

    Plasmid

    #11817

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 8 (CASP8 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKOR1

    Plasmid

    #133446

    Purpose

    Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

    Depositor

    Taeok Bae, Olaf Schneewind

    Article

    Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

    Type

    Empty backbone

    Use

    E.coli/s.aureus shuttle vector

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLG009 (DRT type 1)

    Plasmid

    #157887

    Purpose

    Expresses the DRT type 1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 1 (RT-nitrilase)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLG015 (RADAR)

    Plasmid

    #157893

    Purpose

    Expresses the RADAR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RADAR subtype containing auxillary gene rdrD
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTom20-mScarlet3-H (aka mYongHong)

    Plasmid

    #239412

    Purpose

    Expresses Tom20-mScarlet3-H in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    Tom20 (TOMM20 Human)
    

    Tags

    mScarlet3-H (aka mYongHong)

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA-mCherry-TAG-EGFP

    Plasmid

    #232156

    Purpose

    A fluorescence reporter to show incorporation of target unnatural amino acids.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    mCherry-linker(TAG)-EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT2-shP53

    Plasmid

    #124261

    Purpose

    Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shP53
    

    Expression

    Mammalian

    Available Since

    April 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid

    #178209

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.HA and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge