345,779 results

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pEGFP-ninein

Plasmid

#73519

Purpose

Encodes mouse ninein tagged with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein

Plasmid

#73519

Purpose

Encodes mouse ninein tagged with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

Plasmid

#213026

Purpose

Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag

Tags

3xFlag (ORF2p)

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

Plasmid

#213026

Purpose

Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag

Tags

3xFlag (ORF2p)

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HiBiT-FZD7

Plasmid

#195845

Purpose

Expresses human FZD7 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD7 (FZD7 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD7

Plasmid

#195845

Purpose

Expresses human FZD7 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD7 (FZD7 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti.PGK.blast-Renilla_Luciferase

Plasmid

#74444

Purpose

Control lentiviral vector expressing Renilla luciferase

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Renilla Luciferase

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti.PGK.blast-Renilla_Luciferase

Plasmid

#74444

Purpose

Control lentiviral vector expressing Renilla luciferase

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Renilla Luciferase

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Lapronic/pcDNA3.1(-)

Plasmid

#140756

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lapronic/pcDNA3.1(-)

Plasmid

#140756

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-FLAG-Jhdm2a

Plasmid

#38136

Depositor

Toshinobu Nakamura, Toru Nakano

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-FLAG-Jhdm2a

Plasmid

#38136

Depositor

Toshinobu Nakamura, Toru Nakano

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

pCAG

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

pCAG

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1A-CD19.8H.8TM.28.BB.3z

Plasmid

#200672

Purpose

Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1A-CD19.8H.8TM.28.BB.3z

Plasmid

#200672

Purpose

Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
2HA-pDEST-N

Plasmid

#118373

Purpose

Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
2HA-pDEST-N

Plasmid

#118373

Purpose

Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-FLAG-NAT10-siR-G641E

Plasmid

#59366

Purpose

Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Larrieu et al Science. 2014 May 2;344(6183):527-32. do

Insert

NAT10 NM_024662 (NAT10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

G641E and silent mutations to render the cDNA res…

Promoter

CMV-tet

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-FLAG-NAT10-siR-G641E

Plasmid

#59366

Purpose

Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Larrieu et al Science. 2014 May 2;344(6183):527-32. do

Insert

NAT10 NM_024662 (NAT10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

G641E and silent mutations to render the cDNA res…

Promoter

CMV-tet

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-cytoEKAR4

Plasmid

#174438

Purpose

Cytosol-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

cytoEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-cytoEKAR4

Plasmid

#174438

Purpose

Cytosol-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

cytoEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_LacZ

Plasmid

#25893

Purpose

Gateway entry vector containing LacZ.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

LacZ

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_LacZ

Plasmid

#25893

Purpose

Gateway entry vector containing LacZ.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

LacZ

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-CAG::Mitbow

Plasmid

#158994

Purpose

Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Mitbow

Tags

The default expressed EBFP2 is fused to H2B; oth…

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-CAG::Mitbow

Plasmid

#158994

Purpose

Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Mitbow

Tags

The default expressed EBFP2 is fused to H2B; oth…

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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