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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 32,591 results for: LIS;

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Showing: 9161 - 9200 of 32591 results

  4. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid
    #158576
    Purpose
    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Rudolf Jaenisch
    Article
    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii
    Insert
    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    Tags
    V5 and HA on the endogenous KCC2 protein product
    Expression
    Mammalian
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    158576-plasmid-map-sequence-id-311917

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  11. pLY164

    Plasmid
    #184140
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R3, PrhaB, and Ptet
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184140-plasmid-map-sequence-id-378329

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  12. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197025
    Purpose
    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized RhCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197025-plasmid-map-sequence-id-388959

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  21. pX018

    Plasmid
    #167144
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal luciferase, nnLuz
    Use
    Luciferase and Synthetic Biology
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167144-plasmid-map-sequence-id-335106

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  28. pX019

    Plasmid
    #167145
    Purpose
    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal hispidin-3-hydroxylase, nnH3H
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167145-plasmid-map-sequence-id-335228

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  35. Myc-gamma2S/deltaIL

    Plasmid
    #119730
    Purpose
    GABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Article
    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.
    Insert
    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    119730-plasmid-map-sequence-id-229624

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Showing: 9161 - 9200 of 32591 results