We narrowed to 15,977 results for: LEA

1. WasCFP /pQE-30

   Plasmid

   #79858

   Purpose

   Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

   Insert

   WasCFP
   

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Cyan fluorescent protein mCerulean with substitut…

   Promoter

   T5 promoter / lac operator

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PARP9-c024

   Plasmid

   #173096

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   G102-K298

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TTVTFLPTPSLILQT_epitope

   Plasmid

   #165021

   Purpose

   Expression of CMV UL44 (38-52) fused to CD74

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   CMV UL44 (38-52)
   

   Use

   Lentiviral

   Tags

   CD74 at N-term; cathepsin S cleavage site (GRWHTV…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CMV-evoCjCas9-BlastR

   Plasmid

   #194059

   Purpose

   Mammalian expression of evoCjCas9 nuclease

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEVA421-Cas9tr

   Plasmid

   #138709

   Purpose

   Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   tracrRNA-Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmTurquoise2-NES

   Plasmid

   #36206

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   NES
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PARP15-c020

   Plasmid

   #173101

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP15 (PARP15 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N78-S267, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCVhyg-3xT7-nSREBP1a

    Plasmid

    #171030

    Purpose

    Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

    Depositor

    Kai Ge

    Article

    Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

    Insert

    nSREBP1a (Srebf1 Mouse)
    

    Use

    Retroviral

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    amino acids 1- 449

    Promoter

    5' LTR

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCoofy55

    Plasmid

    #122007

    Purpose

    Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His10-SumoStar (Life Sensors) and Multi2: dependi…

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIVT-dCas9-mCherry

    Plasmid

    #174443

    Purpose

    in vitro transcription template for dCas9-mCherry

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    mCherry

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid

    #216283

    Purpose

    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher003-TEVs-SpyTag003DA
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGCDNsam-Hnf4a

    Plasmid

    #33002

    Depositor

    Atsushi Suzuki

    Article

    Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

    Insert

    hepatic nuclear factor 4 alpha (Hnf4a Mouse)
    

    Use

    Retroviral

    Available Since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpyCatcher002-oDi

    Plasmid

    #124661

    Purpose

    Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oDi
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046768

    Plasmid

    #145763

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-Trpa1b-2A-mCherry

    Plasmid

    #106291

    Purpose

    Gateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Insert

    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway middle entry

    Tags

    2A-mCherry

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193315

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Tags

    4FLAG

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-mCherry-CRY2-NLS

    Plasmid

    #145259

    Purpose

    Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEBTet_NAD-Snifit-nucl

    Plasmid

    #113915

    Purpose

    Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGBW-m4046959

    Plasmid

    #145722

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. SpyCatcher002-oTet

    Plasmid

    #124663

    Purpose

    Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTet
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJ151-HDR

    Plasmid

    #113631

    Purpose

    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

    Depositor

    Alex Kentsis

    Inserts

    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    

    Use

    Cre/Lox; Homology recombination selection vector

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a promoter, EF1a promoter (T2A cleaving sequen…

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pVB3011

    Plasmid

    #113389

    Purpose

    Galactose-inducible L-BC gag: cure killer

    Depositor

    Suzanne Sandmeyer

    Article

    Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

    Insert

    L-BC Gag
    

    Expression

    Yeast

    Mutation

    Please see depositor comments

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Clomeleon

    Plasmid

    #203723

    Purpose

    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

    Depositor

    George Augustine

    Article

    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

    Insert

    cyan fluorescent protein linked yellow florescent protein
    

    Expression

    Bacterial and Mammalian

    Mutation

    Cyan Fluorescent Protein, Topaz Fluorescent Prote…

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SpyCatcher002-oTri

    Plasmid

    #124662

    Purpose

    Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTri
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Venus-StagRFP-AktAR

    Plasmid

    #173020

    Purpose

    Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Venus-StagRFP-AktAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKM3

    Plasmid

    #154168

    Purpose

    Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p5T7-LYCipi-ggpps

    Plasmid

    #122017

    Purpose

    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Insert

    GGPP synthase
    

    Expression

    Bacterial

    Mutation

    codon optimized, truncated first 98 amino acids, …

    Promoter

    T7

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEBTet_NADP-Snifit-nucl

    Plasmid

    #113918

    Purpose

    Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV U6+27

    Plasmid

    #25709

    Purpose

    for nuclear expression of small RNAs in mammalian cells

    Depositor

    David Engelke

    Article

    Good et al Gene Ther. 1997 Jan . 4(1):45-54.

    Insert

    U6 snRNP (LSM2 Human)
    

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. V2-MESA-35F-M-dCas9

    Plasmid

    #84506

    Purpose

    MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-dCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pQL47 EGFP-VAMP-GST

    Plasmid

    #122971

    Purpose

    Reporter of botulinum toxin B cleavage

    Depositor

    Chandra Tucker

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    EGFP-VAMP3-GST
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUAP4

    Plasmid

    #136079

    Purpose

    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pRK1035

    Plasmid

    #8832

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease
    

    Tags

    His and MBP (cleaved)

    Expression

    Bacterial

    Mutation

    synthetic gene with codons optimized for E. coli

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pElectra2-LaminA

    Plasmid

    #179483

    Purpose

    Nuclear envelope labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-LaminA
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBEL1284

    Plasmid

    #175960

    Purpose

    For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Inserts

    LetA
    LetB
    

    Tags

    6xHis 2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMM289

    Plasmid

    #46037

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A/K577M (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M exonuclease and helicase-dead mutant; …

    Promoter

    CMV2

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJBL7044

    Plasmid

    #167215

    Purpose

    overexpression of PbrR in E. coli for cell-free lead sensing

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    PbrR
    

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJBL7061

    Plasmid

    #167230

    Purpose

    cell-free cascaded lead sensor

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pPbr-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMM288

    Plasmid

    #46036

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A exonuclease-dead mutant; S2G introduced duri…

    Promoter

    CMV2

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

47. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCSC-NES-RFP

    Plasmid

    #221812

    Purpose

    Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NES-mCherry
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pre_II_CS3_7653_v2

    Plasmid

    #126855

    Purpose

    7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7653_v2
    

    Use

    Synthetic Biology

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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