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We narrowed to 17,420 results for: She

Showing: 9361 - 9400 of 17420 results

  1. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid
    #216377
    Purpose
    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)
    Depositor
    William Weis
    Insert
    LDL receptor related protein 6 (LRP6 Human)
    Use
    Baculovirus
    Tags
    9xHis and HA signal sequence-FLAG-3C cleavage sit…
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin (LRP6)/p10 (MESD)
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216377-plasmid-map-sequence-id-433174

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  5. pcDNA3-Flag-Tet3 CD Mut

    Plasmid
    #72222
    Purpose
    Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 3
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET3 catalytic domain (cata…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-UbC-mCherry Rab5

    Plasmid
    #115239
    Purpose
    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab5a (RAB5A Homo sapiens, Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human UbC
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115239-plasmid-map-sequence-id-222083

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  16. p-dCas9-LSD1-K661A-Hygro

    Plasmid
    #104414
    Purpose
    transient expression of dCas9-dLSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dLSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104414-plasmid-map-sequence-id-204405

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  17. HTATIP (2OU2)

    Plasmid
    #33338
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP (KAT5 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pcDNA3.1_hMed25

    Plasmid
    #206958
    Purpose
    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.
    Depositor
    Reza Zolfaghari, Catharine Ross
    Insert
    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206958-plasmid-map-sequence-id-422373

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  26. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Use
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  29. sgRNA(MS2)_zeo insert c-MYC

    Plasmid
    #164636
    Purpose
    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
    Depositor
    Günter Schneider
    Insert
    target sequence for c-Myc activation (MYC Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164636-plasmid-map-sequence-id-326094

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  37. pAAV/EF1a-DIO-SCLM

    Plasmid
    #216759
    Purpose
    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    EF1a (human elongation factor 1 alpha)
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    216759-plasmid-map-sequence-id-429631

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  38. AICSDP-144:CTCF-mEGFP

    Plasmid
    #168799
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF
    Depositor
    Allen Institute for Cell Science
    Insert
    CTCF (CTCF Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168799-plasmid-map-sequence-id-334694

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  39. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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Showing: 9361 - 9400 of 17420 results