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Showing: 9721 - 9760 of 160711 results

  1. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  3. pENTR-GFP-MDC1 (770-7)

    Plasmid
    #26284
    Depositor
    Eric Campeau
    Insert
    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    Use
    Entry vector
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26284-plasmid-map-sequence-id-12370

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  6. human ACF

    Plasmid
    #112860
    Purpose
    plasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 complementation factor (A1CF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112860-plasmid-map-sequence-id-224730

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  9. pCMV-Luc

    Plasmid
    #45968
    Depositor
    Ralf Kuehn
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45968-plasmid-map-sequence-id-64565

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  10. pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 (AAV9)

    Viral Prep
    #124650-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 (#124650). In addition to the viral particles, you will also receive purified pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 plasmid DNA. CamKIIa-driven expression of C1V1(t/t) fused to mScarlet fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Harvey
    Article
    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-
    Promoter
    CaMKII
    Tags
    mScarlet
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154867-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  18. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid
    #223505
    Purpose
    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223505-plasmid-map-sequence-id-443299

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  23. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

    Viral Prep
    #112677-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  28. PB CAG-tdTomato

    Plasmid
    #133569
    Purpose
    CAG tdTomato piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133569-plasmid-map-sequence-id-274028

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Showing: 9721 - 9760 of 160711 results