163,421 results

1. pcDNA3-GFPLaminA-L85R

   Plasmid

   #32707

   Depositor

   Wendy Bickmore

   Article

   Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

   Insert

   Lamin A (LMNA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L85R

   Promoter

   CMV

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. AiP12558 - pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2558)

   Plasmid

   #208141

   Purpose

   AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

   Plasmid

   #199359

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOEu-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p415-GalL-Cas9-CYC1t

   Plasmid

   #43804

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GalL

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBFP2-C1

   Plasmid

   #22880

   Depositor

   Dorus Gadella

   Article

   Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

   Insert

   SBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBad-UGAcS

   Plasmid

   #172137

   Purpose

   bacteria expression plasmid for a UDP-GlcNAc sensor

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

   Insert

   UGAcS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H605Q- please see depositor comments

   Promoter

   araBAD

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. XRE-pNL1.3[secNLuc]

   Plasmid

   #182295

   Purpose

   Secreted Luciferase reporter for AhR activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   Xenobiotic Response Element (CYP1A1 Human)
   

   Use

   Tags

   secNluc

   Expression

   Mammalian

   Mutation

   Promoter

   XRE

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-NpHR-EYFP

   Plasmid

   #171621

   Purpose

   TRE-NpHR-EYFP reporter

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TRE-NpHR-EYFP
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-UGAcS

    Plasmid

    #172135

    Purpose

    mammalian expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

    Plasmid

    #164184

    Purpose

    Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3_cAMPFIRE-H

    Plasmid

    #182281

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI-Neo HA-hSki

    Plasmid

    #10910

    Depositor

    Bob Weinberg

    Article

    Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    Ski (SKI Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-CAG-M-SPOTIT2

    Plasmid

    #191489

    Purpose

    Green fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    M-SPOTIT2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3 3 kb prom. CD274

    Plasmid

    #107004

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-nEF-Coff/Fon-ChRmine-oScarlet (AAV8)

    Viral Prep

    #137160-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChRmine-oScarlet (#137160). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Flp (inhibited in the presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    oScarlet (Flp-dependent)

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

18. HA-RNF4/pRK5

    Plasmid

    #59743

    Purpose

    Expresses RNF4 in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM60 FLAG-RAB35 Q67L

    Plasmid

    #107105

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-PPO-mScarlet

    Plasmid

    #139503

    Purpose

    Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Use

    Tags

    mScarlet following Gly-Gly-Ser linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lyric-mNeonGreen

    Plasmid

    #226562

    Purpose

    Encodes Lyric protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJEC780

    Plasmid

    #234349

    Purpose

    Expressed synthetic RNA target for RENDR

    Depositor

    James Chappell

    Article

    Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

    Insert

    Synthetic RNA target for RENDR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLC-mCherry-MLKL

    Plasmid

    #75167

    Purpose

    LentiCRISPR-mCherry with sgRNA targeting human MLKL

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    MLKL sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CaMKIIa-EGFP (AAV2)

    Viral Prep

    #50469-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    EGFP

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

30. eGFPC1 FKBP

    Plasmid

    #67529

    Purpose

    Expresses GFP fused FKBP

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Insert

    FKBP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F36V

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RF13

    Bacterial Strain

    #62078

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCR1307

    Plasmid

    #111133

    Purpose

    Expresses WDR48.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    WDR48 (WDR48 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDP20

    Plasmid

    #171097

    Purpose

    Expresses mAID-tagged mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    mCherry2
    

    Use

    Synthetic Biology

    Tags

    miniAID

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCold TDP43 M337V

    Plasmid

    #27453

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Use

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pSP64-hKCNE1

    Plasmid

    #53049

    Purpose

    Expresses beta subunit for IKs channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENN.AAV.CamKII 0.4.Cre.SV40

    Plasmid

    #105558

    Purpose

    AAV expression of Cre from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. phGluc

    Plasmid

    #22522

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLJM60 FLAG-RAB35 S22N

    Plasmid

    #107106

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Galphai2-mTurquoise2

    Plasmid

    #69621

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai2-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge