167,924 results

1. pmCherry-MYRIP

   Plasmid

   #89586

   Purpose

   Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYRIP (Myrip Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOSIP-KO (KanR, 186)

   Plasmid

   #45985

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMMP-MCS-IRES-eGFP

   Plasmid

   #36953

   Depositor

   Bill Sugden

   Article

   Kennedy et al Mol Cell Biol. 2003 Oct;23(19):6901-8.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-hChR2-H134R-tdTomato

   Plasmid

   #28017

   Purpose

   AAV-mediated expression of hChR2(H134R) for neuronal excitation.

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Has Service

   AAV Retrograde

   Insert

   humanized ChR2-H134R-tdTomato
   

   Use

   AAV; Adeno associate viral

   Tags

   tdTomato

   Mutation

   H134R

   Promoter

   CAG

   Available Since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL20_mEGFP_PAX7_FOXO1

   Plasmid

   #205891

   Purpose

   Express mEGFP-tagged fusion protein, PAX7_FOXO1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   PAX7_FOXO1 (PAX7, FOXO1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-X1-Neo-HA-SEC62

   Plasmid

   #139865

   Purpose

   Lentiviral expression of HA-tagged human SEC62

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   SEC62 (SEC62 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist GFP-Linker-VDAC1

   Plasmid

   #211735

   Purpose

   Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

   Depositor

   Roland Malli

   Article

   Pilic et al bioRxiv 2023.09.08.556841

   Insert

   Voltage-dependent anion channel 1 (VDAC1 )
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV mClover3-Galectin-3

   Plasmid

   #215376

   Purpose

   Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   LGALS3 (LGALS3 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid

    #216153

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic splice site and C-terminal FLAG tag
    

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL SFFV d20GFP.T2A.mTagBFP Donor

    Plasmid

    #31485

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d20BFP T2A BFP
    

    Use

    Lentiviral

    Mutation

    GFP has 20 amino acids deleted from the C terminu…

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. LeGO-V2

    Plasmid

    #27340

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, Venus fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

    Plasmid

    #233629

    Purpose

    Expression of bicistronic reporter with mutated TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Mutation

    AGAAGG in TOP motif

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKm-HGBE

    Plasmid

    #170334

    Purpose

    a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized GBEs
    

    Use

    CRISPR

    Mutation

    Cas9n-D10A

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Expression

    Mammalian

    Promoter

    Tight TRE promoter and U6

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTcI42-Wasabi

    Plasmid

    #86118

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI42
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, K6N, S33T, Y61H, L119P, F122C

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWALIUM10-GW-TurboID-V5

    Plasmid

    #196283

    Purpose

    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Insect

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKm-HABE

    Plasmid

    #170332

    Purpose

    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized ABEs
    

    Use

    CRISPR

    Mutation

    Cas9n-D10A

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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24. R777-E035 Hs.DUSP2

    Plasmid

    #70319

    Purpose

    Gateway ORF clone of human DUSP2 [NM_004418.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    DUSP2 (DUSP2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-Ef1a-fDIO mCherry (AAV5)

    Viral Prep

    #114471-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Flp-dependent)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

26. pEGFPC1-EPSIN 1

    Plasmid

    #22228

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    please see depositor comment below

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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27. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pTiger

    Plasmid

    #1728

    Purpose

    This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system

    Depositor

    Garry Nolan

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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29. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    HIS and MYC

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLZ12Km2-P23R:TA:fflucRT

    Plasmid

    #88901

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + Red-shifted firefly luciferase
    

    Expression

    Bacterial

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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