167,948 results

1. IRES-mCherry

   Plasmid

   #80139

   Purpose

   empty retroviral vector with IRES-mCherry

   Depositor

   Ellen Rothenberg

   Article

   Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CL20_mEGFP_HEY1_NCOA2

   Plasmid

   #205829

   Purpose

   Express mEGFP-tagged fusion protein, HEY1_NCOA2 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   HEY1_NCOA2 (HEY1, NCOA2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-NES-CRY2PHR-TevC

   Plasmid

   #89877

   Purpose

   expresses NES-CRY2PHR-TevC of BLITz system

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   NES-CRY2PHR-TevC
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGIP

   Plasmid

   #26777

   Purpose

   Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   EF1a-GFP-IRES-Puro (gfp )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpRY dCas9 KRAB

   Plasmid

   #188501

   Purpose

   Expresses FLAG tagged SpRY dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-IRES-Thy1.1 DEST

   Plasmid

   #17442

   Purpose

   Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSFFV_sfCherry2_TagBFP

   Plasmid

   #83031

   Purpose

   Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry+full length_TagBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   3 mutations in sfCherry full length: E118Q, T128I…

   Promoter

   SFFV

   Available Since

   Jan. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV-C 18E6

   Plasmid

   #37884

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 18E6 (E6 HPV)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1_PAsfCherry2(1-10)

   Plasmid

   #82612

   Purpose

   Express PAsfCherry2(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   PAsfCherry2(1-10)
   

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   CMV

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid

    #239102

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Donor plasmid for integration into the human geno…

    Tags

    p2A-tdTomato-p2A-Thy1.2

    Expression

    Mammalian

    Promoter

    Endogenous POU4F2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mH2A1.2-CT-MYC

    Plasmid

    #45168

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    HIS and MYC

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUltraI-Tet3.0[TAA]

    Plasmid

    #164580

    Purpose

    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    

    Expression

    Bacterial

    Mutation

    L170G, N311G, C313A

    Promoter

    ProK and TacI

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mcherry-Myo 9b

    Plasmid

    #134918

    Purpose

    Expression of rat Myo 9b (Myr 5) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

    Insert

    Myo 9b (rat) with 3' UTR (Myo9b Rat)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-ERBB2-S310F

    Plasmid

    #116349

    Purpose

    Lentiviral expression of ERBB2 S310F

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Mutation

    S310F

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UAS:iGluSnfr.zf1

    Plasmid

    #108356

    Purpose

    UAS driven variant of iGluSnFR for zebrafish expression

    Depositor

    Harold Burgess

    Article

    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

    Insert

    UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
    

    Use

    Zebrafish expression

    Promoter

    lac

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. gh69

    Plasmid

    #106740

    Purpose

    expression of gRNA targeting B3GNT3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GNT3 (B3GNT3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p6641 MSCV-P C-FlagHA 18E7-Kozak

    Plasmid

    #35019

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV18 E7 (E7 HPV 18)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    March 14, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-hSyn-Cre-P2A-dTomato (AAV5)

    Viral Prep

    #107738-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Syn

    Tags

    dTomato (physically separate, not a fusion protein)

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

24. pCOLA-T7-WspR

    Plasmid

    #79162

    Purpose

    Expresses WspR WT under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid

    #174722

    Purpose

    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    eGFP
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and F2A linker

    Expression

    Mammalian

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAC-GAL4

    Plasmid

    #24344

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Expression

    Insect

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSBbi G418R mbIL21

    Plasmid

    #194329

    Purpose

    Consitiutive mammalian expression of membrane bound human IL-21

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    mbIL-21 (IL21 Human)
    

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge