264,297 results

1. p3-K230-FokI_L (DAS)

   Plasmid

   #32546

   Depositor

   Jin-Soo Kim

   Article

   Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

   Insert

   ZFN
   

   Use

   Tags

   HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

2. p3-K230-FokI_R (RR)

   Plasmid

   #32547

   Depositor

   Jin-Soo Kim

   Article

   Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

   Insert

   ZFN
   

   Use

   Tags

   HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pHES262 [prADH1-Msn2-mCherry, pnh604]

   Plasmid

   #103844

   Purpose

   Yeast Expression Plasmid

   Depositor

   Hana El-Samad

   Article

   Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

   Insert

   pAdh1-Msn2-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

   Plasmid

   #117910

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A on eDHFR

   Promoter

   T7

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1-mCherry-Nanobody

   Plasmid

   #70696

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   mCherry-Nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-IRES-mAmetrine 1.1 FP

   Plasmid

   #52113

   Purpose

   a bicistronic IRES-mAtetrine containing retroviral vector

   Depositor

   Dario Vignali

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mAmetrine 1.1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-hM4D(Gi)-mCherry

   Plasmid

   #50477

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry (CHRM4 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

   Plasmid

   #116885

   Purpose

   FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RVH)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGfa(B3)-nLac

   Plasmid

   #53132

   Purpose

   expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells

   Depositor

   Michael Brenner

   Article

   de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   -2163 to +47, with 3 copies of the gfa2 B segment…

   Promoter

   human Gfa2

   Available since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3xFLAG-Tmprss6(S762A)

    Plasmid

    #18791

    Depositor

    Bruce Beutler

    Article

    Du et al Science. 2008 May 23. 320(5879):1088-92.

    Insert

    Type II transmembrane serine protease 6 (Tmprss6 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Serine762 to Alanine

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. FepA

    Plasmid

    #26039

    Depositor

    Johann Deisenhofer

    Article

    Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

    Insert

    FepA (fepA E Coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pFA6a-hphMX6-81nmt1

    Plasmid

    #105164

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-81nmt1
    

    Use

    Tags

    81nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    81nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-hphMX6-41nmt1

    Plasmid

    #105163

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-41nmt1
    

    Use

    Tags

    41nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1177

    Plasmid

    #29091

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple81 (GAL Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB-Bleo Gal4 tBFP

    Plasmid

    #139190

    Purpose

    Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.

    Depositor

    Neal Devaraj

    Insert

    tBFP
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Gal4 CMV and STAT3

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. mScarlet-PPM1H

    Plasmid

    #233585

    Purpose

    Expresses PPM1H in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

    Plasmid

    #230989

    Purpose

    Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Membrane-localized Voltron2 and soma-localized CheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

    Plasmid

    #230990

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5_mCherry-P300

    Plasmid

    #238258

    Purpose

    For overexpression of mCherry-P300

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGM117

    Plasmid

    #220168

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    CISD1 (CISD1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. GFP-Kv2.2

    Plasmid

    #231678

    Purpose

    Expression of mouse Kcnb2 fused with EGFP in C-terminal.

    Depositor

    Federico Sesti

    Insert

    Kcnb2 (Kcnb2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KHC068 BRD2-KI-GFP-2A-L387A

    Plasmid

    #231713

    Purpose

    CRISPR mediated KI of BromoTag at N-terminus of BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065

    Depositor

    Alessio Ciulli

    Article

    Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

    Insert

    Brd2 (BRD2 Human)
    

    Use

    CRISPR

    Tags

    BromoTag and GFP

    Expression

    Mammalian

    Mutation

    L387A

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPB_EF1a_HA-tNGFR_PuroR

    Plasmid

    #212652

    Purpose

    piggyBac vector to express HA-tNGFR with puromycin resistance

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    tNGFR
    

    Use

    Piggybac

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p5E-CAGGS (JDW 912)

    Plasmid

    #224472

    Purpose

    Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    CAGGS Promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

    Plasmid

    #224490

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-iRFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p5E-EF1a (JDW 1164)

    Plasmid

    #224473

    Purpose

    Gateway compatible 5' entry clone containing the human EF1a promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NFAST(low)-mApple-mito

    Plasmid

    #214415

    Purpose

    Mitochondria localization of low-affinity NFAST fused to mApple-mito

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-mito

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CFAST-Halotag-6xHp

    Plasmid

    #214416

    Purpose

    Lipid droplet localization of CFAST fused to Halotag-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    deletion
    

    Use

    Tags

    CFAST-Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR223-BILF1

    Plasmid

    #195797

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BILF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BILF1 (BILF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR223-BILF2

    Plasmid

    #195788

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BILF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BILF2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge