163,297 results

1. pJET-CMV-GFP-P2A-K0-P2A-mCherry

   Plasmid

   #185619

   Purpose

   Ribosomal stalling reporter: empty

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   GFP-empty-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJET-CMV-GFP-P2A-I15-P2A-mCherry

   Plasmid

   #185620

   Purpose

   Ribosomal stalling reporter: 15x isoleucine

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   GFP-I15-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-mGFAP-Lck-PinkFlamindo

   Plasmid

   #228394

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

   Insert

   Pink Flamindo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   mGFAP

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIB-mRuby3-STX17TM

   Plasmid

   #221744

   Purpose

   Stable expression of mRuby3-STX17TM in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   STX17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-228

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDC180

   Plasmid

   #218673

   Purpose

   Expresses rat ⍺SNAP in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   rat ⍺SNAP (Napa Rat)
   

   Use

   Tags

   6 his

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4.23_Promoter_4

   Plasmid

   #210630

   Purpose

   Luciferase experiment for TAL1 promoter 4

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   promoter 4 (TAL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23_exon 4

   Plasmid

   #210631

   Purpose

   Luciferase experiment for TAL1 exon 4

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1 exon 4 (TAL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Promoter

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF1606

   Plasmid

   #143829

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BAZ2A (BAZ2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1717

   Plasmid

   #142834

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LDB1 (LDB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTR-340 pUC19-tNGFR-P2A-IL2RG N

    Plasmid

    #186067

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RG (IL2RG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-mCherry-PA-Rac1

    Plasmid

    #169042

    Purpose

    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    PA-Rac-1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Gateway entry cloning vector

    Tags

    6X His and Fused with mCherry

    Expression

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

    Plasmid

    #164013

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R464E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a human Tankyrase2 full length Y920E

    Plasmid

    #132639

    Purpose

    expresses human Tankyrase2 full length Y920E in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

    Insert

    Tankyrase2 Y920E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ninaE-norpA

    Plasmid

    #26854

    Depositor

    Craig Montell

    Article

    Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

    Insert

    no receptor potential A (norpA Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V963A

    Promoter

    Available since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDKN1A sgRNA

    Plasmid

    #138189

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDKN1A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBABE-zeo largeTgenomic

    Plasmid

    #1778

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T and small T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lamp1-mCherry-FRB

    Plasmid

    #186576

    Purpose

    Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    mCherry-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Human J-chain

    Plasmid

    #158215

    Purpose

    Express the human J-chain

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    JCHAIN (JCHAIN Human)
    

    Use

    Tags

    8×his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. evoBE4-NG

    Plasmid

    #226852

    Purpose

    Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBUN421

    Plasmid

    #62204

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    TaU3 and Ubi

    Available since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-KO-Puro

    Plasmid

    #227262

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR_TetO_CD19-P2A-mCherry

    Plasmid

    #133803

    Purpose

    repressible expression of CD19 and mCherry in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    putative ECD of H. sapiens CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p6618 MSCV-IP N-HAonly WHSC2

    Plasmid

    #35014

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    WHSC2 (NELFA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

29. Str-Ii_VSVG-SBP-mCherry

    Plasmid

    #65301

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

31. p5-71

    Plasmid

    #118085

    Purpose

    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

    Depositor

    Bryan Dickinson

    Article

    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

    Insert

    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCas3-Amp

    Plasmid

    #207998

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBoBi-hLAMP2-C-GC6s

    Plasmid

    #154151

    Purpose

    To detect the calcium ion releases close to the lysosomal surface

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

    Insert

    LAMP2 and GCamp6s (LAMP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. HA-EglN2-pcDNA3

    Plasmid

    #18961

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 2 (EGLN2 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

    Plasmid

    #205631

    Purpose

    Expression of TnpBmax in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB optimized for mammalian expression
    

    Use

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCL-CTIG

    Plasmid

    #14901

    Purpose

    3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

    Depositor

    Inder Verma

    Article

    Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

    Insert

    CMV-tTA-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FLT3-MSCV-IRES-GFP

    Plasmid

    #184778

    Purpose

    Expresses wildtype FLT3 in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCherryC1 VAP-A KD/MD

    Plasmid

    #226408

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-A (VAPA Human)
    

    Use

    Tags

    T7-Xpress tag and mCherry

    Expression

    Mammalian

    Mutation

    K94D/M96D mutant, unable to bind FFAT motifs

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 SARS-CoV-2 E

    Plasmid

    #158080

    Purpose

    Expresses SARS-CoV-2 envelope protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 envelope (E )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge