167,785 results

1. NbV5-eGFP

   Plasmid

   #201473

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-eGFP
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0675

   Plasmid

   #141659

   Purpose

   Lentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM16 (PRDM16 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ALK5 T204D

   Plasmid

   #80877

   Purpose

   mammalian expression of constitutively active ALK5 T204D

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T204D (constitutively active)

   Promoter

   CMV

   Available Since

   Aug. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

4. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. JMJD1BA-c068

   Plasmid

   #53627

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD1BA
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. GB1-A11(2-196)-Strep

   Plasmid

   #196208

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTn7-SCOUT11.G

   Plasmid

   #200999

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   superfolder GFP
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF525-EF1a-Hygro-P2A-mCherry-lenti

   Plasmid

   #115796

   Purpose

   Expresses mCherry in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EF-1a promoter

   Available Since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE:GFP

   Plasmid

   #163036

   Purpose

   FLiCRE reporter gene for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   GFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVS1

    Plasmid

    #33143

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Insert

    LacO (256 copies)
    

    Expression

    Yeast

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 p53 WT

    Plasmid

    #69003

    Purpose

    mammalian expression of wild type human p53

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ211_rha_WT_low

    Plasmid

    #128851

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0372

    Plasmid

    #141593

    Purpose

    Lentiviral vector for overexpressing the NFYB transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFYB (NFYB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6/V5-DEST-SPAG5

    Plasmid

    #31217

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    SPAG5 (SPAG5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Contains a one nucleotide deletion at C-term that…

    Available Since

    Aug. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

16. PS-CFP2-MyosinIIA-C-18

    Plasmid

    #57241

    Purpose

    Localization: Cytoskeleton, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    MyosinIIA (Myh9 Mouse)
    

    Tags

    PS-CFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Tol2-zTRAP

    Plasmid

    #42236

    Depositor

    Stephen L Johnson

    Article

    Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

    Insert

    EGFP-Rpl10a (rpl10a Zebrafish)
    

    Tags

    EGFP

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    CaMKIIa

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pooled sgRNA libraries Set 1 and Set 2 for Pseudomonas aeruginosa PA14

    Pooled Library

    #1000000254

    Depositor

    Xue Liu

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

20. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. TGFB1-bio-His

    Plasmid

    #52185

    Purpose

    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TGFB1 (TGFB1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. WT GLUT1-FLAG

    Plasmid

    #200094

    Purpose

    WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. HA-Sestrin2 (E451Q)

    Plasmid

    #237420

    Purpose

    Transient expression of N-terminally HA-tagged human Sestrin2 (E451Q)

    Depositor

    David Sabatini

    Article

    Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

    Insert

    Sestrin2 (SESN2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E451Q

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

    Plasmid

    #238181

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pVeBtA2

    Plasmid

    #238987

    Purpose

    Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p3VEA3

    Plasmid

    #234796

    Purpose

    Mammalian expression of bovine arrestin-3

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid

    #231552

    Purpose

    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    F-tractin (Itpka Rat)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid

    #231556

    Purpose

    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pKanB

    Plasmid

    #223523

    Purpose

    Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. P1K-3HA-Atg8∆R-MET15

    Plasmid

    #207034

    Purpose

    Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆R

    Promoter

    pATG8

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TFORF2476

    Plasmid

    #144030

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFHX2 (ZFHX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pD649-HAsp-CD70-COMP5AP-AviTag-9xHis

    Plasmid

    #157113

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD70 (CD70 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFRTpsiCHECK_SEAP

    Plasmid

    #154461

    Purpose

    Expression of SEAP and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    SEAP
    

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. H2B-HT-emiRFP703

    Plasmid

    #183985

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Tags

    HaloTag, emiRFP703

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145262

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+8A)-IDR (HOXD13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHAGE-EPHA2-G391R

    Plasmid

    #116320

    Purpose

    Lentiviral expression of EPHA2 G391R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Lentiviral

    Mutation

    G391R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge