215,785 results

1. ClCry4 PHR

   Plasmid

   #160552

   Purpose

   Expression of protein for various experiments including finding interaction partners

   Depositor

   Joseph Takahashi

   Article

   Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

   Insert

   Clcry4 HR
   

   Tags

   Hexa his

   Expression

   Insect

   Available Since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Actinin-sstFRET-GR

   Plasmid

   #83416

   Purpose

   FRET-based actinin tension sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

   Insert

   Actinin-sstFRET-GR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

3. AAVS1-TRE-SHH

   Plasmid

   #134350

   Purpose

   Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

   Depositor

   Lorenz Studer

   Article

   Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

   Insert

   SHH (SHH Human)
   

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG.PKCepsilon.K/W

   Plasmid

   #10796

   Depositor

   Alex Toker

   Article

   Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

   Insert

   PKC epsilon (PRKCE Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K437W (Lys 437 to Trp)

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

7. FLAG.PKCepsilon.K/W

   Plasmid

   #10796

   Depositor

   Alex Toker

   Article

   Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

   Insert

   PKC epsilon (PRKCE Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K437W (Lys 437 to Trp)

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

8. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

   Plasmid

   #170209

   Purpose

   Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGCaMP6s-P2A-H2AmCherry

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICE-HA-NLS-I-PpoI

   Plasmid

   #46963

   Purpose

   For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   NLS-I-PpoI
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV-TET

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-TET

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmal_c5x_RNAse_Inhib

    Plasmid

    #153314

    Purpose

    Bacterial expression of an RNase inhibitor

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    RNAse Inhibitor
    

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB533-44B12m23-sfGFP

    Plasmid

    #186778

    Purpose

    RNAP2 Ser5ph-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    44B12m23-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC-NRP1

    Plasmid

    #158384

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    NRP1 (NRP1 Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAF0474 Ef1a-Si74_attB acceptor

    Plasmid

    #193475

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKD3_mCherry

    Plasmid

    #187385

    Purpose

    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-NL1(-)

    Plasmid

    #15260

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 (Nlgn1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-NL1(-)

    Plasmid

    #15260

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 (Nlgn1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. ER-mEGFP

    Plasmid

    #182876

    Purpose

    Localization: Endoplasmic Reticulum

    Depositor

    Salvatore Chiantia

    Article

    Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

    Insert

    ER-mEGFP
    

    Expression

    Mammalian

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. AAV-CamKIIa-jGCaMP8m-WPRE (AAV5)

    Viral Prep

    #176751-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CamKIIalpha

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

23. Fuw-dCas9-Tet1CD-P2A-BFP

    Plasmid

    #108245

    Purpose

    Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A

    Promoter

    Ubc

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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30. myc-KIF5B

    Plasmid

    #127617

    Purpose

    N-terminal myc-tagged mouse KIF5B

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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32. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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34. pLenti-CMV-NFE2L2-Puro

    Plasmid

    #181919

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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35. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Expression

    Mammalian

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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36. pCAG-NLS-HA-Dre

    Plasmid

    #51272

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Dre
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-NLS-HA-Dre

    Plasmid

    #51272

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Dre
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCFJ601 - Peft-3 Mos1 transposase

    Plasmid

    #34874

    Purpose

    Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Mos1 transpoase
    

    Use

    Worm targeting

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCFJ601 - Peft-3 Mos1 transposase

    Plasmid

    #34874

    Purpose

    Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Mos1 transpoase
    

    Use

    Worm targeting

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    pTet

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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