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Showing: 781 - 810 of 190729 results

  1. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62201-plasmid-map-sequence-id-228092

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  2. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

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  3. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

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  4. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  5. pAAV-hSyn-DIO-mCherry (AAV5)

    Viral Prep
    #50459-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. pGP-AAV-syn-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162374-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

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  14. pAAV-hSyn-GRAB_ACh3.0 (AAV9)

    Viral Prep
    #121922-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_ACh3.0 (#121922). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_ACh3.0 plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s
    Promoter
    Syn
    Tags
    None
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

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  16. pAAV-flex-taCasp3-TEVp (AAV5)

    Viral Prep
    #45580-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nirao Shah, Jim Wells
    Article
    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104993-plasmid-map-sequence-id-342099

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  23. pSAB35

    Plasmid
    #175787
    Purpose
    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag
    Depositor
    John Rubinstein
    Article
    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246
    Insert
    SidK (1-278)
    Use
    Tags
    3xFLAG and 6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175787-plasmid-map-sequence-id-348166

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  24. pLEX-MCS UBC promoter

    Plasmid
    #128045
    Purpose
    Lentiviral vector with UBC promoter
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    128045-plasmid-map-sequence-id-256398

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Showing: 781 - 810 of 190729 results