We narrowed to 2,435 results for: LRE

1. HaloTag-ER-3

   Plasmid

   #186960

   Purpose

   ER lumen localizaing HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CALR (CALR Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

   Plasmid

   #213398

   Purpose

   transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

   Depositor

   Katrin Willig

   Article

   Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

   Insert

   FingR for endogenous PSD95, transcriptinally regulated
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV MCL1 Puro

   Plasmid

   #140746

   Purpose

   Expresses MCL1 in mammalian cells

   Depositor

   Scott Dixon

   Article

   Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

   Insert

   MCL1 (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-PD-1-SNAP

   Plasmid

   #223576

   Purpose

   Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-PD-L1-SNAP

   Plasmid

   #223577

   Purpose

   Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFR-FR

   Plasmid

   #179263

   Purpose

   Human EGFR C-terminally labeled with FusionRed

   Depositor

   Jared Toettcher

   Article

   Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   FusionRed

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DIO-oG-WPRE-hGH

   Plasmid

   #74290

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK287 (mAID-Hygro)

   Plasmid

   #72825

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Hygro
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-TetOn-dCas9-KRAB

    Plasmid

    #115545

    Purpose

    Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid

    #68375

    Purpose

    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK1

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS1479-HTP-KanMX6

    Plasmid

    #154085

    Purpose

    For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

    Depositor

    Lidia Vasiljeva

    Article

    Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

    Insert

    his6-TEV-protein A-KanR
    

    Use

    Pcr-based tagging in yeast

    Tags

    his6-TEV-protein A

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. L1-neo-TET

    Plasmid

    #51284

    Purpose

    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    L1RE1 (L1RE1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAP2

    Plasmid

    #73211

    Purpose

    express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    AAP2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19_CRISPR_DpmrA

    Plasmid

    #160903

    Purpose

    CRISPR_Cas9, lambda red recombineering

    Depositor

    Anne-Catrin Uhlemann

    Article

    McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

    Inserts

    Cas9
    Lambda Red Recombineering Genes
    Zeocin Resistance Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMy-BirA-T2A-eGFP

    Plasmid

    #50565

    Purpose

    Retroviral vector encoding for GFP and the biotinylating enzyme BirA

    Depositor

    Rafael Casellas

    Article

    Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

    Insert

    Ampicilin
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

    Plasmid

    #62897

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAGM55261

    Plasmid

    #153210

    Purpose

    Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. FLAG-Cdk9 T186A

    Plasmid

    #28101

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Contains a T186A mutation in Cdk9

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetroX-PTuner DD-linker-M.EcoGII

    Plasmid

    #122082

    Purpose

    Express M.EcoGII with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of M.EcoGII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUCC001

    Plasmid

    #124451

    Purpose

    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HH-BsaI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    gRNA expression cassette from pUDP002 modified to…

    Promoter

    TDH3 promoter from S.cerevisiae (already present …

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCAGGS_VP30_MARV

    Plasmid

    #135472

    Purpose

    Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    VP30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. PGK 4xMito-mEmerald

    Plasmid

    #200430

    Purpose

    Mammalian expression of fluorescently-tagged marker for mitochondria

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    4x mito-targeting sequence

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FLAG-Cdk9

    Plasmid

    #28100

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge