169,450 results
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Plasmid#118354PurposeThis plasmid expresses human MEF2A-1 WT.DepositorAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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piRFP670-N1
Plasmid#45457DepositorHas ServiceCloning Grade DNAInsertiRFP670
ExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF)
Pooled Library#1000000113PurposeGenome-wide CRISPR gRNA pooled library for activation of mouse genesDepositorAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
ExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir2-LNS2
Plasmid#220086PurposeC-terminal PA binding domain of Nir2DepositorInsertPITPNM1 (PITPNM1 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 896-1216PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX
Plasmid#208692PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Endosomes-14
Plasmid#58014PurposeLocalization: Endosomes, Excitation: 555, Emission: 584DepositorAvailable SinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Factor IX donor
Plasmid#182141PurposeFactor IX donor for twinPE and Bxb1 mediated insertion at ALBDepositorInsertattP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
ExpressionMammalianAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas9-Act3.0
Plasmid#178954PurposeIt consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertzCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163043PurposeQuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseLentiviralTagsmOrange2ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
GUM BUB3
Plasmid#84029PurposeExpression of mCherry tagged BUB3 in human cellsDepositorAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB094a
Plasmid#79241PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4; PC araC
ExpressionBacterialPromoterPBAD; PCAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-eYFP
Plasmid#104056PurposeAn AAV genome with tet-inducible expression of the fluorescent protein eYFPDepositorInsertEYFP
UseAAVPromoterTREAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ucp1-Cre-miR122
Plasmid#228408PurposeExpression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.DepositorInsertscre recombinase with SV40 NLS
Cre recombinase with SV40 NLS
UseAAVPromoterrat Ucp1 enhancer-promoterAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianPromoterEF1aAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 WT
Plasmid#27451DepositorAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
Tagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-W105M-IRES-mCherry
Plasmid#68857PurposeExpresses Human CDK8 W105M MutantDepositorInsertCyclin-Dependent Kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pcDNA6)
Plasmid#26578DepositorAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF3039
Plasmid#144515PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-Ruby.W
Plasmid#51009PurposeLentivector that expresses LifeAct-Ruby from internal human PGK promoterDepositorInsertLifeAct-mRFPruby
UseLentiviralTagsLifeActExpressionMammalianPromoterhuman PGKAvailable SinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralExpressionMammalianPromoterEF1Alpha/TRE3GAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsFLAG tagExpressionMammalianPromoterCAG promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b_mNeonGreen
Plasmid#209425PurposeExpress mNeonGreen in E. coliDepositorInsertmNeonGreen
Tags6xHisExpressionBacterialAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR_PGK_antiCD19_H1_Gal4VP64
Plasmid#169918PurposeExpression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.DepositorInsertantiCD19-Notch1-GL4VP64
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-N1
Plasmid#54595PurposeLocalization: N1 Cloning Vector, Excitation: 383, Emission: 448DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsEBFP2ExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRX-IP-mGFP-NCOA4
Plasmid#192802PurposeStable expression of mGFP-NCOA4 in mammalian cellsDepositorAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only