163,403 results

1. lentiCRISPR-v2-HygR-EGFP

   Plasmid

   #167188

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-FLAG-NAT10-siR-WT

   Plasmid

   #59365

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Larrieu et al Science. 2014 May 2;344(6183):527-32. do

   Insert

   human NAT10 (NAT10 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Mutations to render cDNA resistant to a siRNA

   Promoter

   CMV-tet

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

   Plasmid

   #231986

   Purpose

   Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   OVA-2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187080

   Purpose

   Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

5. pMemoryArray

   Plasmid

   #60585

   Purpose

   An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   MemoryArray
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Brachyury-eGFP

   Plasmid

   #21063

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Brachyury promoter
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV SYN PSAM4 GlyR IRES EGFP (AAV5)

   Viral Prep

   #119742-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from AAV SYN PSAM4 GlyR IRES EGFP (#119742). In addition to the viral particles, you will also receive purified AAV SYN PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Promoter

   Syn

   Tags

   EGFP

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH0001

   Plasmid

   #85969

   Purpose

   UCOE-SFFV-dCas9-BFP-KRAB

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BFP-KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   SFFV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_047

    Plasmid

    #107145

    Purpose

    measures Cas9 activity

    Depositor

    David Root

    Insert

    GFP + sgRNA for GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINTO-N3::hTET3

    Plasmid

    #136364

    Purpose

    Expresses epitope-tagged human TET3 WT in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Use

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-SMYD2

    Plasmid

    #85658

    Purpose

    retroviral expression of human SMYD2

    Depositor

    Julien Sage

    Article

    Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

    Insert

    SMYD2 (SMYD2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7C-HSPA8

    Plasmid

    #177661

    Purpose

    Recombinant protein synthesis of human HSPA8

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T-1 3xFlag-p38alpha

    Plasmid

    #47575

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    p38alpha (Mapk14 Mouse)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCAG:GPI-GFP

    Plasmid

    #32601

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Rhee et al Genesis. 2006 Apr;44(4):202-18.

    Insert

    GPI-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe.puro.CDK8.flag

    Plasmid

    #19758

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. TL026_AAV_hSyn:SEP-GluA1

    Plasmid

    #192519

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    AAV and Cre/Lox

    Tags

    super ecliptic phluorin

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A-GFP-N2 (FR1)

    Plasmid

    #19364

    Purpose

    Gateway entry vector for C-term eGFP fusion, frame N2

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Flag-Tet2 CD

    Plasmid

    #72219

    Purpose

    Express flag-tagged mouse Tet2 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 2 (Tet2 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET2 catalytic domain.

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET30-HPF1-His-Sumo-Flag

    Plasmid

    #111577

    Purpose

    Expresses full length HPF1 in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    HPF1 (HPF1 Human)
    

    Use

    Tags

    6xHis-Sumo-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-tag

    Plasmid

    #55180

    Purpose

    FLAG-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    FLAG
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-Flag HBx

    Plasmid

    #42596

    Depositor

    Ding Xue

    Article

    Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

    Insert

    Hepatitis B virus protein X
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. DNA repair library with 1,573 sgRNAs

    Pooled Library

    #177663

    Purpose

    CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

27. XE251 pCDNA3.1(zeo)-hDsh3

    Plasmid

    #16758

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6-CENP-A-mCherry

    Plasmid

    #89767

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CENP-A (CENPA Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAB2076 pAAV REPAIR.t1

    Plasmid

    #176323

    Purpose

    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    

    Use

    AAV and CRISPR

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    EFS (short EF1alpha) and hU6

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWPI-SLC7A11-HA

    Plasmid

    #201643

    Purpose

    Over-expression of HA-tagged human SLC7A11

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Lenti_MCP-p300_Hygro

    Plasmid

    #138459

    Purpose

    lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    p300 core
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-EGFP-Cdc42(wt)

    Plasmid

    #12599

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (WT) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-TetON-3G

    Plasmid

    #96963

    Purpose

    Mammalian expression of Clontech rtTA driven by human beta-actin promoter

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV5-Smad7-HA

    Plasmid

    #11733

    Depositor

    Jeff Wrana

    Article

    Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

    Insert

    SMAD7 (SMAD7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pIVTR(T7A)-NGN2 (SA Substitutions)

    Plasmid

    #172308

    Purpose

    In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)

    Depositor

    Patrik Verstreken

    Insert

    NGN2
    

    Use

    Other

    Tags

    Expression

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A an…

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p5-71

    Plasmid

    #118085

    Purpose

    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

    Depositor

    Bryan Dickinson

    Article

    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

    Insert

    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLVX-Puro-TDP-43-WT

    Plasmid

    #133753

    Purpose

    Wild type human TDP-43 in pLVX-Puro lentiviral vector

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge