We narrowed to 8,450 results for: set

1. pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

   Plasmid

   #111390

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA-mRuby2
   

   Use

   AAV

   Tags

   Myc and mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mTHC

   Plasmid

   #112620

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Human, Anthozoa)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Tags

   mTeal (mTFP1)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_S.AU1.VSVg_NGFR

   Plasmid

   #158351

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   S.AU1.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLH_Scr16

   Plasmid

   #100538

   Purpose

   CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

   Inserts

   Phytochrome B (PHYB Mustard Weed)
   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Cdc1 NLS, CreC, CreN, and SV40 NLS

   Expression

   Yeast

   Mutation

   AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A

   Promoter

   FBA1 and TDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

   Plasmid

   #111391

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   ChETA-mRuby2
   

   Use

   AAV

   Tags

   Myc and mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

   Plasmid

   #156452

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

   Depositor

   Elphege Nora

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   RAD21, AID (Rad21 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSin-EF2-DMD53-GFP

   Plasmid

   #138353

   Purpose

   Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences

   Depositor

   Baisong Lu

   Article

   Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do

   Insert

   DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   EF2

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO_V5.AU1.VSVg_NGFR

   Plasmid

   #158241

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5.AU1.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

    Plasmid

    #111392

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA-EYFP
    

    Use

    AAV

    Tags

    EYFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAav-TP53-T2A-BirA*

    Plasmid

    #115652

    Purpose

    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

    Inserts

    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    

    Use

    AAV

    Tags

    BirA* and T2A

    Expression

    Mutation

    Homology region 1 and Homology region 2

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. ptdTHC

    Plasmid

    #112617

    Purpose

    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Promoterless vector, ready for promoter for mamma…

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_HA.V5.VSVg_NGFR

    Plasmid

    #158244

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO_NWS.AU1.VSVg_NGFR

    Plasmid

    #158239

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    NWS.AU1.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO_HA.AU1.VSVg_NGFR

    Plasmid

    #158232

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.AU1.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEXSISERS_FLuc_loxP-PuroR

    Plasmid

    #177866

    Purpose

    Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    FLuc-based EXSISERS
    

    Use

    CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVHC

    Plasmid

    #112614

    Purpose

    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Promoterless vector, ready for promoter for mamma…

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_V5.AU1.FLAG_NGFR

    Plasmid

    #158240

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5.AU1.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_NWS.V5.VSVg_NGFR

    Plasmid

    #158246

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    NWS.V5.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_V5.FLAG.VSVg_NGFR

    Plasmid

    #158247

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5.FLAG.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLKO_NWS.V5.FLAG_NGFR

    Plasmid

    #158251

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    NWS.V5.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO_S.V5.HA_NGFR

    Plasmid

    #158339

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.V5.HA

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO_Ollas.AU1.C_NGFR

    Plasmid

    #158314

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.AU1.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Drd1a->M71-IRES-tauGFP ACNF TV

    Plasmid

    #105073

    Purpose

    Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette

    Depositor

    Peter Mombaerts

    Article

    Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

    Insert

    Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Drd1a coding sequence followed by IRES-tauGFP ACNF

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO_HA.AU1.V5_NGFR

    Plasmid

    #158235

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.AU1.V5

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGG369

    Plasmid

    #165606

    Purpose

    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

    Expression

    Bacterial

    Mutation

    Recoding of BsaI site in the AmpR cassette, two B…

    Promoter

    lacUV2 driving ω-dCas9 and UV5 driving sgRNA

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAJ1

    Plasmid

    #138379

    Purpose

    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ

    Depositor

    Seth Darst

    Article

    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11

    Inserts

    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…

    Promoter

    T7

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. cftr_R (OZ518)

    Plasmid

    #27193

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting cftr (cftr Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pCAG-EHC

    Plasmid

    #112619

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEHC

    Plasmid

    #112615

    Purpose

    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Promoterless vector, ready for promoter for mamma…

    Tags

    Emerald

    Expression

    Mammalian

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge