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Showing: 8431 - 8460 of 161041 results

  2. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

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  9. AAV-EF1a-jGCaMP8m-WPRE (AAV1)

    Viral Prep
    #176757-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-EF1a-jGCaMP8m-WPRE (#176757). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8m-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    EF1a
    Tags
    None
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  14. kiCAP-AAV-MyoAAV1E

    Plasmid
    #224439
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDTMSK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224439-plasmid-map-sequence-id-446666

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  15. pSBtet-GP

    Plasmid
    #60495
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60495-plasmid-map-sequence-id-237935

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  19. pEGFP-BLM

    Plasmid
    #110299
    Purpose
    Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase
    Depositor
    Chris Kok-Lung Chan
    Insert
    BLM (Bloom's Syndrome) helicase (BLM Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    110299-plasmid-map-sequence-id-211197

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  25. xPERT-hA2q

    Plasmid
    #231063
    Purpose
    Circularly permuted CasRx platform fused to human deaminase domain for A to I editing
    Depositor
    Meng How Tan
    Article
    Wang et al Nat Biotechnol. 2024 Oct 9. doi: 10.1038
    Inserts
    dCas13d
    Human deaminase ADAR2dd (ADARB1 Ruminococcus flavefaciens XPD3002)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H460D, E488Q, only the deaminase domain (aa 276-7…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231063-plasmid-map-sequence-id-459741

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  26. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  28. pcDNA3.1-MBII85-5'SS mut

    Plasmid
    #67631
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.
    Depositor
    Stefan Stamm
    Article
    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00
    Insert
    MBII85 snoRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutation in 5'ss
    Promoter
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67631-plasmid-map-sequence-id-116897

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Showing: 8431 - 8460 of 161041 results