167,318 results

1. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. C1-F-tractin-mCherry

   Plasmid

   #155218

   Purpose

   Monitor global F-actin distribution

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138130

   Purpose

   Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-mouse-IgG(kappa)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL20_mEGFP_FGFR3_TACC3

   Plasmid

   #205821

   Purpose

   Express mEGFP-tagged fusion protein, FGFR3_TACC3 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   FGFR3_TACC3 (FGFR3, TACC3 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB2-mScarlet-I

   Plasmid

   #104007

   Purpose

   Plasmid encoding mScarlet-I

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mScarlet-I
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-deadMalibu-mScarlet

   Plasmid

   #229555

   Purpose

   Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

   Depositor

   Danielle Schmitt

   Article

   Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

   Insert

   Malibu-mScarlet
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. siRNA-resistant mNG-ORP8

   Plasmid

   #195170

   Purpose

   mammalian expression of siRNA-resistant ORP8 tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ORP8 (OSBPL8 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from K174 t…

   Promoter

   CMV

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-NOP56-C3

   Plasmid

   #134536

   Purpose

   Expresses YFP-NOP56 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   NOP56 (NOP56 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-ZDHHC5

    Plasmid

    #231654

    Purpose

    Expresses human ZDHHC5 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC5 (ZDHHC5 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRGLO Dual-Luciferase-PDAP1-3'UTR

    Plasmid

    #182251

    Purpose

    Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV2)

    Viral Prep

    #105547-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pQE80L-IL2 C125S

    Plasmid

    #104348

    Purpose

    Expresses human IL-2 (C125S)

    Depositor

    Alexander McLellan

    Insert

    Human IL-2 (C125S) (IL2 Human)
    

    Tags

    MRGSHHHHHGS-tag

    Expression

    Bacterial

    Mutation

    C125S mutation (TGT -> TCC)

    Promoter

    T5

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-EGFP-GRAM-H

    Plasmid

    #211703

    Purpose

    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Fusion protein with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Lifeact-7

    Plasmid

    #54491

    Purpose

    Localization: Action, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mCherry-Lifeact

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbE8a-RFP

    Plasmid

    #35268

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmCherry-2xFYVE

    Plasmid

    #140050

    Purpose

    Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. modRNAc1-VP64dCas9VP64-2A-MPH

    Plasmid

    #216388

    Purpose

    this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    VP64dCas9VP64-2A-MPH
    

    Use

    CRISPR

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Promoter

    rat Tyrosine hydroxylase

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-EF1a-DIO-JEDI-2P-WPRE (AAV1)

    Viral Prep

    #179460-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-WPRE (#179460). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-WPRE plasmid DNA. EF1a-driven, Cre-dependent expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Promoter

    EF1a

    Tags

    cEGFP (Cre-dependent)

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

28. pBbS8a-RFP

    Plasmid

    #35274

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pR-spondin 1

    Plasmid

    #128844

    Purpose

    bacterial expression of pR-spondin 1

    Depositor

    Marc de la Roche

    Article

    Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

    Insert

    R-spondin 1 (RSPO1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDNR-mCherry-T2A-3xPuroR-Frame 0

    Plasmid

    #206991

    Purpose

    Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge