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175,750 results

Showing: 11281 - 11320 of 175750 results

  1. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid
    #174659
    Purpose
    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    Xon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174659-plasmid-map-sequence-id-351621

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  2. pSBtet-GB

    Plasmid
    #60504
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60504-plasmid-map-sequence-id-97543

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  3. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV2)

    Viral Prep
    #100054-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CAG
    Tags
    mCherry
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  4. pRN120

    Plasmid
    #160696
    Purpose
    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator
    Depositor
    Daniel Voytas
    Article
    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103
    Inserts
    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    Use
    Luciferase; Gemini viral replicon, plant developm…
    Promoter
    AtUbi10, CmYLCV, and Nos
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160696-plasmid-map-sequence-id-316629

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  5. pAAV-hSynapsin- soCoChR-GFP (AAV9)

    Viral Prep
    #107708-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin- soCoChR-GFP (#107708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin- soCoChR-GFP plasmid DNA. Synapsin-driven somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.
    Promoter
    Syn
    Tags
    GFP
    Available Since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pEXPqcxip-hFTSJ3-FLAG

    Plasmid
    #165463
    Purpose
    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.
    Depositor
    Martin Dorf
    Article
    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11
    Insert
    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    Use
    Retroviral; Gateway
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV/MSV, 5'LTR
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    165463-plasmid-map-sequence-id-327804

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  7. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

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  8. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55112-plasmid-map-sequence-id-257392

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  9. GFP-ESR1

    Plasmid
    #65211
    Purpose
    Full length mammalian expression vector for hESR1
    Depositor
    Ratna Vadlamudi
    Insert
    esr1 (ESR1 Human)
    Tags
    AcGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65211-plasmid-map-sequence-id-422716

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  13. pVP143

    Plasmid
    #222080
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222080-plasmid-map-sequence-id-438352

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  16. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  18. pLIX403-EBFP2-ccdB-G418

    Plasmid
    #158556
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158556-plasmid-map-sequence-id-309869

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  20. pVITRO1-102.1F10-IgA1/λ

    Plasmid
    #50370
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50370-plasmid-map-sequence-id-75287

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  24. pCfB2223

    Plasmid
    #67544
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67544-plasmid-map-sequence-id-116393

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  26. pAG426-GAL-Alpha-synuclein

    Plasmid
    #181715
    Purpose
    Galactose inducible expression of Alpha-synuclein
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Expression
    Yeast
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    181715-plasmid-map-sequence-id-436188

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  27. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    51295-plasmid-map-sequence-id-339890

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  31. SPLICSFAST-LDh-LY-P2A

    Plasmid
    #214895
    Purpose
    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-LY-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214895-plasmid-map-sequence-id-423481

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  32. pLenti-rInsp-hIns-GLuc

    Plasmid
    #89927
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    410bp rat insulin II promoter
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    89927-plasmid-map-sequence-id-255126

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Showing: 11281 - 11320 of 175750 results