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167,785 results

Showing: 11281 - 11320 of 167785 results

  1. pAAV.hSyn.CaMPARI.WPRE.SV40 (AAV1)

    Viral Prep
    #100832-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.CaMPARI.WPRE.SV40 (#100832). In addition to the viral particles, you will also receive purified pAAV.hSyn.CaMPARI.WPRE.SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter, Loren Looger
    Article
    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d
    Promoter
    Syn
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  2. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #162382-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  4. Mito-B

    Plasmid
    #209864
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209864-plasmid-map-sequence-id-415402

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  5. TLCV2-LoxP

    Plasmid
    #127098
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system.
    Depositor
    Adam Karpf
    Insert
    Cas9-2A-eGFP
    Use
    Lentiviral
    Promoter
    U6
    Available Since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127098-plasmid-map-sequence-id-246495

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  7. PB-FLuc+GFPd2

    Plasmid
    #127190
    Purpose
    Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)
    Depositor
    Jordan Green
    Insert
    Firefly luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127190-plasmid-map-sequence-id-248023

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  10. rApoI

    Bacterial Strain
    #156459
    Purpose
    An E. coli strain that expresses rat APOBEC1. Used as a control strain for mutagenesis experiments with MutaT7.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Bacterial Resistance
    Streptomycin
    Species
    Rattus norvegicus
    Available Since
    Oct. 19, 2020
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-Ef1a-Con/Foff 2.0-EYFP (AAV8)

    Viral Prep
    #137162-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-EYFP (#137162). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-EYFP plasmid DNA. EF1a-driven expression of EYFP in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  17. pTP943

    Plasmid
    #104157
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fab specific nanobody TP886
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104157-plasmid-map-sequence-id-200077

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  20. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187241-plasmid-map-sequence-id-374769

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  23. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid
    #212022
    Purpose
    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    GianCreg (GOLGB1 Human)
    Tags
    td5StayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212022-plasmid-map-sequence-id-419221

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  24. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  27. RH2b-bio

    Plasmid
    #47778
    Purpose
    Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH2b (partial)
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47778-plasmid-map-sequence-id-68797

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  31. ER-M-TAG (pGS555)

    Plasmid
    #160040
    Purpose
    pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    MMGT1-mScarlet-3xHA
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160040-plasmid-map-sequence-id-323176

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  34. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  39. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

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Showing: 11281 - 11320 of 167785 results