We narrowed to 17,061 results for: UTY
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Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP1FBAR(516a.a.)-EGFP
Plasmid#168505PurposeExpression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.DepositorInsertSRGAP1-FBAR (SRGAP1 Human)
UseTagsEGFPExpressionMammalianMutationaa 1-516PromoterCAGAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB088i
Plasmid#79216PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB085c
Plasmid#79208PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); PtetAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-HA-EGFP-Myc-TM-IRES-Pac
Plasmid#183605PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHA, Human PDGFRB transmembrane domain, Mouse IgGK…ExpressionMammalianMutationPromoterCAGAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-Ku70siR-WT
Plasmid#46961PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman Ku70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMV TetAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BD-cop1at(335-675)
Plasmid#44973DepositorInsertBD-COP1 (COP1 Mustard Weed)
UseTagsGAL4 DNA-binding domainExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMVAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB088c
Plasmid#79213PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJKW1813
Plasmid#174003PurposeExpresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorInsertEGFP
UseTagsHuman EMD transmembrane domain and NLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB076i6
Plasmid#79200PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (no operator); Ppro1Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB085d7
Plasmid#79211PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB085d6
Plasmid#79210PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
EW265 SFFV-mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP (FLP-IN)
Plasmid#236124PurposePlasmid encoding fluorescent reporter for PUF-9R binding which expresses mCherry constitutively and EGFP upon stop codon deamination due to ADAR-PUF9R bindingDepositorInsertmCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)
Plasmid#233538PurposeA CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion proteinDepositorInsertLEF1 (LEF1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)
Plasmid#233542PurposeA CMV promoter driving expression of a murine dominant negative myc tagged TCF4.DepositorInsertTCF4 (TCF4 Mouse)
UseLentiviralTagsMycExpressionMutationlacks aa 1-56PromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)
Plasmid#232128PurposeA middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorInsertGATA2 (GATA2 Human)
UseGateway cloningTagsV5ExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)
Plasmid#232123PurposeA middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorInsertLEF1 (LEF1 Human)
UseGateway cloningTagsV5ExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)
Plasmid#232129PurposeA middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorInsertMAFB (MAFB Human)
UseGateway cloningTagsV5ExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)
Plasmid#232131PurposeA middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorInsertFOXF2 (FOXF2 Human)
UseGateway cloningTagsV5ExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)
Plasmid#232130PurposeA middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorInsertGATA4 (GATA4 Human)
UseGateway cloningTagsV5ExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB1
Plasmid#162691PurposeExpresses DabAB1 inorganic carbon transport complexDepositorInsertDabAB1 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB051e
Plasmid#79195PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPExt-10cons lambda op-62 gIII, luxAB; Ptet lambda cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPExt-10cons lambda op-62; PtetAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAB078d7
Plasmid#79205PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (off-target); Ppro1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pAB085e
Plasmid#79212PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F7
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); PtetAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL-CD86
Plasmid#183244PurposeExpression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsertCD86, 41BBL
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10APromoterj23119 and ptbAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY125 (pcDNA3.1-huBfCpf1)
Plasmid#92301PurposeExpression of humanized BfCpf1DepositorInserthuBfCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY124 (pcDNA3.1-huBsCpf1)
Plasmid#92300PurposeExpression of humanized BsCpf1DepositorInserthuBsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHPMP190 (pLifeAct tagRFP-T)
Plasmid#88842PurposeLocalization by Fluorescence microscopy of lifeAct fused to tagRFP-TDepositorInsertLifeAct
UseTagstagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFG018
Plasmid#62817PurposeBacterial inducible gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’
Plasmid#162703PurposepCB reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutation5513T>G (tetR E37A); 7758G>T (second tet op…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
UseTagsExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
RSV-Flag-Brd2
Plasmid#86614PurposeFlag tagged mouse Brd2 expression construct for cell transfectionDepositorInsertBrd2 (Brd2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterRSVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RSV-Flag-Brd4
Plasmid#86616PurposeFlag tagged mouse Brd4 expression construct for cell transfectionDepositorInsertBrd4 (Brd4 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterRSVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
RSV-Flag-Brd3
Plasmid#86615PurposeFlag tagged mouse Brd3 expression construct for cell transfectionDepositorInsertBrd3 (Brd3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterRSVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVAX1-BMP2
Plasmid#137909PurposeConstitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNADepositorInsertBMP2 (BMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Pansio
Plasmid#214441PurposeBxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-HA-EGFP-Myc-TM-IRES-Pac
Plasmid#183606PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHA, Human PDGFRB transmembrane domain, Mouse IgGK…ExpressionMammalianMutationPromoterMouse Pgk1Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
UseTagsBFP, flagExpressionYeastMutationPromoterTDH3Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)
Plasmid#233549PurposeA CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.DepositorInsertGATA4 (GATA4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Keppel
Plasmid#214444PurposeBxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Olônne
Plasmid#214443PurposeBxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE
Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorInsertMKK6 (MAP2K6 Human)
UseTagsExpressionBacterialMutationSer207 to Glu, Thr211 to GluPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only