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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,046 results for: Nes;

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Showing: 11601 - 11640 of 26046 results

  2. pSicoR PGK puro

    Plasmid
    #12084
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12084-plasmid-map-sequence-id-169379

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  7. pVS2

    Plasmid
    #33144
    Depositor
    Michael Rosbash
    Article
    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.
    Inserts
    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    Expression
    Yeast
    Mutation
    GFP is mutated such that amino acids 64-73 (FSYGV…
    Promoter
    bidirectional GAL1-GAL10 promoter
    Available Since
    Dec. 20, 2011
    Availability
    Academic Institutions and Nonprofits only

  8. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. M8

    Plasmid
    #180253
    Purpose
    Vaccine vector encoding 8 neonatigens specific for the MC38 colon cancer mouse model
    Depositor
    Fabio Palombo
    Article
    Salvatori et al NPJ Vaccines. 2022 Feb 2;7(1):15. doi: 1
    Insert
    Synthetic gene encoding for 8 neonatigens specific for MC38 mouse cancer cells
    Use
    Vaccine vector
    Expression
    Mammalian
    Mutation
    the polyepitope vaccine encodes for mutated seque…
    Promoter
    CMV
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    180253-plasmid-map-sequence-id-357261

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  20. pRSF_AaRbcLS

    Plasmid
    #229505
    Purpose
    Expresses Anthoceros agrestis Rubisco large and small subunit (C terminus hexahistidine)
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco large subunit
    Rubisco small subunit
    Tags
    hexahistidine
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229505-plasmid-map-sequence-id-456757

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  31. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  38. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al iScience. 2026 Feb 2;29(3):114875. doi:
    Type
    Empty backbone
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Available Since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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  39. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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Showing: 11601 - 11640 of 26046 results