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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 581 - 600 of 16276 results

  1. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid
    #127358
    Purpose
    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127358-plasmid-map-sequence-id-246304

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  4. EBFP2-N1

    Plasmid
    #54595
    Purpose
    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    EBFP2
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    20 Articles
    54595-plasmid-map-sequence-id-364695

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  7. eeBxb1

    Plasmid
    #222339
    Purpose
    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells
    Depositor
    David Liu
    Publication
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222339-plasmid-map-sequence-id-473210

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  8. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid
    #46168
    Purpose
    Codon optimized Cas9_SV40 NLS with intron
    Depositor
    John Calarco
    Publication
    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized Cas9_SV40 NLS with intron
    Use
    CRISPR
    Mutation
    intron inserted at position 3113-3163, and SV40 N…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    July 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    46168-plasmid-map-sequence-id-65793

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  9. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    62202-plasmid-map-sequence-id-288285

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  12. pETM33_Nsp5_Mpro

    Plasmid
    #156475
    Purpose
    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    156475-plasmid-map-sequence-id-307727

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  13. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid
    #73498
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    73498-plasmid-map-sequence-id-364403

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Showing: 581 - 600 of 16276 results