167,910 results

1. pcDNA3-HA-BHLHE40

   Plasmid

   #110154

   Purpose

   Expression of human BHLHE40 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_NSlmb-vhhGFP4

   Plasmid

   #35579

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSlmb-vhhGFP4 (slmb Fly)
   

   Expression

   Mammalian

   Available Since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-H2B tdTomato

   Plasmid

   #116870

   Purpose

   Nuclear localized tdTomato

   Depositor

   Loren Looger

   Article

   Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

   Insert

   H2B tdTomato
   

   Use

   AAV

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-fDIO EYFP (AAV8)

   Viral Prep

   #55641-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   mouse oxytocin

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AMPK(T>A)-SPARK

   Plasmid

   #226256

   Purpose

   phospho-null mutant of AMPK-SPARK

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   AMPK(T>A)-SPARK
   

   Expression

   Mammalian

   Mutation

   substituted Threonine 20 with Alanine

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC-TFRC

    Plasmid

    #172420

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEZT-BM

    Plasmid

    #74099

    Purpose

    Multi-purpose rapid titer bacmam expression vector

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Bacmam, baculovirus

    Tags

    EGFP following p10 promoter

    Expression

    Mammalian

    Promoter

    CMV, p10

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_mCherry_P2A_Hygro

    Plasmid

    #135003

    Purpose

    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ALDH18A1

    Plasmid

    #155402

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (AAV5)

    Viral Prep

    #209655-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (#209655). In addition to the viral particles, you will also receive purified pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag plasmid DNA. CAG-driven, Cre-dependent expression of the high affinity ATP biosensor iATPSnFR2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Promoter

    CAG

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

17. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV and CMV (IRES)

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJet-CMV-IARS1-GFP

    Plasmid

    #212322

    Purpose

    EGFP-tagged IARS1

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    IARS (IARS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.22-VEGF-HRE::dLUC

    Plasmid

    #128096

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Promoter

    inserted VEGF hypoxia response elements (HRE) (x5)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. Cav1-GFP

    Plasmid

    #14433

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. pIVT-eeBxb1

    Plasmid

    #232748

    Purpose

    IVT template for making modRNA encoding eeBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Mutation

    V74A, E229K, V375I

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-hSyn-DIO-GRAB-gDA3m

    Plasmid

    #208707

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. AiP13775 - pAAV-AiE0050m_3xC2-minBG-iCre-BGHpA (Alias: CN3775)

    Plasmid

    #230379

    Purpose

    AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre
    

    Use

    AAV

    Promoter

    minRho

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLEX-307_LgBiT

    Plasmid

    #191211

    Purpose

    Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    LgBiT (large bit)
    

    Use

    Lentiviral and Luciferase

    Mutation

    AB030246.1 (mutated in PMID 26569370)

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid

    #216668

    Purpose

    Expresses membrane-bound mCherry in mammalian cells

    Depositor

    Leonardo Morsut

    Insert

    mCherry
    

    Tags

    PDGFR Transmembrane Domain

    Expression

    Mammalian

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RGB-S reporter

    Plasmid

    #207841

    Purpose

    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

    Depositor

    Christof Niemeyer

    Article

    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

    Inserts

    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    

    Expression

    Bacterial

    Promoter

    PosmY, PsulA, and grpE

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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