We narrowed to 150,751 results for: ins

1. RRRAA-5-mut-pHG165c

   Plasmid

   #170113

   Purpose

   Constitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

   Depositor

   Liskin Swint-Kruse

   Article

   Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

   Insert

   RRRAA-5-MUT
   

   Expression

   Bacterial

   Mutation

   5A14L, W116Q, E178K, S184T, S237L

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

   Plasmid

   #159664

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMS22: pHelper(PmcCAST)_entry_ΔTniQ

   Plasmid

   #168155

   Purpose

   Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

   Plasmid

   #168152

   Purpose

   Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS5: pHelper(AvCAST)_entry_ΔTniQ

   Plasmid

   #168138

   Purpose

   Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMS4: pHelper(AvCAST)_entry

   Plasmid

   #168137

   Purpose

   Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

   Plasmid

   #168134

   Purpose

   Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-MIOX only

   Plasmid

   #166683

   Purpose

   Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   GFP-MIOX
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTEF1-UDH

   Plasmid

   #166679

   Purpose

   Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   UDH
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. wtVP1 only

    Plasmid

    #166673

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

    Plasmid

    #159656

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KG#797

    Plasmid

    #110916

    Purpose

    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Tags

    GFP (contains 3 artificial introns)

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCBH-Cas9-Suntag-BB_BbsI

    Plasmid

    #164805

    Purpose

    REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

    Plasmid

    #159829

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

    Plasmid

    #159833

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 908I

    Plasmid

    #160241

    Purpose

    VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Van Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIS-rPALcc

    Plasmid

    #145804

    Purpose

    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Expression

    Mammalian

    Mutation

    changed serine 767 to alanine to prevent glycosyl…

    Promoter

    CMV

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

    Plasmid

    #133795

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge