We narrowed to 28,294 results for: cmv promoter

Showing: 9931 - 9960 of 28294 results

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

Plasmid

#187575

Purpose

Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF1 (SRSF1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OT1.0

Plasmid

#185384

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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RUSH reporter-HMGB1-SBP-GFP

Plasmid

#172357

Purpose

Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

Depositor

Guido Kroemer

Article

Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

Insert

high mobility group box 1 (HMGB1 Human)

Use

Lentiviral

Tags

SBP-GFP

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61β

Plasmid

#172445

Purpose

Expression of ER protein Sec61beta with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Irf7

Plasmid

#178445

Purpose

doxycycline-inducible expression of Mouse Irf7 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Irf7 (Irf7 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-mito

Plasmid

#174180

Purpose

Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

AcGFP with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Drp1

Plasmid

#110946

Purpose

Expresses dynamin-like protein 1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Insert

Dynamin-1-like protein (DNM1L Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKRaION1-N1

Plasmid

#177801

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION1

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Smad3

Plasmid

#172098

Purpose

Expresses fluorescently tagged human Smad3 in mammalian cells.

Depositor

Lea Goentoro

Article

Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Smad 3 (Smad3 Mouse)

Tags

mNeonGreen

Expression

Mammalian

Mutation

C49S mutation in mCerulean

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMEH365 Hspa8 HA

Plasmid

#168219

Purpose

Mammalian expression of heat shock 70 kDa protein 8 fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Hspa8

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMEH343 CD63 MS2 HA

Plasmid

#168217

Purpose

Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

CD63 (CD63 Human)

Tags

HA and MS2 dimer

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Lambda

Plasmid

#177579

Purpose

expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Lambda variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-UGAcS

Plasmid

#172135

Purpose

mammalian expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-AMBRA1-3xFLAG

Plasmid

#174157

Purpose

Vector for expression of 3xFLAG-tagged wild-type AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173870

Purpose

Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mNFAT4-462:TALE:VP64:KRɸ

Plasmid

#160662

Purpose

Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

Depositor

Roman Jerala

Article

Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

Insert

mNFAT4-462:TALE:VP64:KRɸ

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP2

Plasmid

#172322

Purpose

an improved fluorescent biosensor for thioredoxin redox

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

TrxRFP2, an improved version from TrxRFP1

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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opto-RhoGEF-mCherry_pcDNA3.1

Plasmid

#164473

Purpose

Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

Insert

opto-RhoGEF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma4-GFP2

Plasmid

#166776

Purpose

Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma4-GFP2 (GNG4 Synthetic, Human)

Expression

Mammalian

Mutation

Contains an N-terminal GFP2 and GSAG linker

Promoter

CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-LmnB1

Plasmid

#164249

Purpose

retroviral plasmid for GFP-LmnB1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

LMNB1 (LMNB1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPPAT-mCherry

Plasmid

#167162

Purpose

Expresses human PPAT-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Pedley et al Methods Mol Biol. 2018;1764:279-289. doi

Insert

PPAT (PPAT Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pmiCas9

Plasmid

#162758

Purpose

express miCas9

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

Contains Brex27 sequence range is 4958-5065

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT6

Plasmid

#164697

Purpose

Expression of PRMT6 with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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