167,846 results

1. pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

   Plasmid

   #239948

   Purpose

   Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   Fc2-MFSD6(L3)
   

   Expression

   Bacterial and Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc-C Med12His

   Plasmid

   #49240

   Purpose

   expresses human Med12 with His tag in insect cells

   Depositor

   Dylan Taatjes

   Article

   Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

   Insert

   Med12 (MED12 Human)
   

   Tags

   His

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLUK

   Plasmid

   #64181

   Purpose

   harbours marker genes HIS3, LEU2, URA3 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT120b

   Plasmid

   #122604

   Purpose

   BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   gIII, luxAB, guide RNA
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-GBF1

   Plasmid

   #206415

   Purpose

   Mammalian expression of hGBF1

   Depositor

   Kazuhisa Nakayama

   Article

   Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

   Insert

   GBF1 (GBF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKD-SepRS-EFSep

   Plasmid

   #34623

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Inserts

   SepRS (MMP0688 Methanocaldococcuc maripaludis)
   EF-Sep (tufB E. coli)
   

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac and Ptac (from SepRS- operon like)

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide-Puro-P2A-EGFP_mRFPstuf

   Plasmid

   #137730

   Purpose

   Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T-1 3xHA-MEK1-DD

    Plasmid

    #47576

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MEK1-DD (Map2k1 Mouse)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser218 to Asp, Ser221 to Asp

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pU6_rd6_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232433

    Purpose

    pegRNA with optimized 3' modifications to correct the rd6 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG2ab

    Plasmid

    #80616

    Purpose

    pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMB-SaCas9

    Plasmid

    #105998

    Purpose

    SaCas9 expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Insert

    Cas9 nuclease
    

    Use

    CRISPR and Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    short EF1alpha

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230019

    Purpose

    AAV vector expressing anti-mCherry PAGER(Gi) in neurons

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-hEAAT1

    Plasmid

    #32813

    Depositor

    Susan Amara

    Insert

    EAAT1 (SLC1A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. RXFP1-Tango

    Plasmid

    #66492

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP1 (RXFP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLV_Zika_NS3_Flag

    Plasmid

    #79635

    Purpose

    Third generation Self-inactivating lentivector expressing NS3 from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    NS3
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_SOX10_P2A_Hygro_Barcode

    Plasmid

    #120479

    Purpose

    Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE3G-GSX2

    Plasmid

    #96964

    Purpose

    Dox-inducible expression of Gsx2 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 (Gsx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TroponinT-GCaMP5-Zeo

    Plasmid

    #46027

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GCaMP5-2A-Zeo
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TNNT2

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221-SLC17A7_STOP

    Plasmid

    #161126

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC17A7 (SLC17A7 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pDONR221_SLC12A9

    Plasmid

    #131897

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC12A9 (SLC12A9 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pFL-EGFP-3xFlag-HLTF

    Plasmid

    #193055

    Purpose

    Baculovirus expression of human HLTF for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    HLTF (HLTF Human)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pGL2-Basic-Myogenin core promoter

    Plasmid

    #134722

    Purpose

    Myogenin core promoter in a luciferase reporter plasmid

    Depositor

    Michael Chin

    Article

    Sun et al J Biol Chem. 2001 May 25;276(21):18591-6

    Insert

    Myogenin Promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    Myogenin

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCW57.1_V5_FLAG_Ct

    Plasmid

    #194075

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-V5

    Expression

    Mammalian

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGL3-ELAM-luc

    Plasmid

    #13029

    Depositor

    Doug Golenbock

    Insert

    ELAM (SELE Human)
    

    Use

    Luciferase

    Tags

    Luc

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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28. pILGFPI7

    Plasmid

    #83555

    Purpose

    Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CUP1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDONR221-SLC32A1_STOP

    Plasmid

    #161278

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC32A1 (SLC32A1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid

    #208959

    Purpose

    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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