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We narrowed to 4,526 results for: GCA

Showing: 1361 - 1400 of 4526 results

  4. ATP1A1_G3_EBFP_Nick_Dual_sgRNA

    Plasmid
    #178094
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + EBFP nick sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178094-plasmid-map-sequence-id-350137

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  12. pSC24

    Plasmid
    #104798
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter
    Depositor
    Nevin Young
    Article
    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.
    Insert
    Medtr2g101120, Medtr2g101130
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    104798-plasmid-map-sequence-id-202699

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  15. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  16. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    125689-plasmid-map-sequence-id-271202

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  17. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  21. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid
    #100025
    Purpose
    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    V5 Epitope Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100025-plasmid-map-sequence-id-194289

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  22. pPBC-G6s

    Plasmid
    #62812
    Purpose
    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62812-plasmid-map-sequence-id-123114

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  24. pPBC-G6f

    Plasmid
    #62811
    Purpose
    pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62811-plasmid-map-sequence-id-123116

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  25. pET28a-HaloTag7SS-DogTag

    Plasmid
    #171775
    Purpose
    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    HaloTag7SS-DogTag
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    DogTag inserted in HaloTag7 between residues D139…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171775-plasmid-map-sequence-id-338524

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  27. pPBC-LG6s

    Plasmid
    #62809
    Purpose
    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62809-plasmid-map-sequence-id-123119

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  29. pPBC-LG6f

    Plasmid
    #62808
    Purpose
    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    62808-plasmid-map-sequence-id-123121

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  30. pPBC-G3-tdT

    Plasmid
    #62814
    Purpose
    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62814-plasmid-map-sequence-id-121226

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  33. pRSET a MatryoshCaMP6s

    Plasmid
    #100019
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100019-plasmid-map-sequence-id-194295

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Showing: 1361 - 1400 of 4526 results