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Plasmid#133025PurposeExpresses EGFP-tagged tail anchor sequence of monoamine oxidase ADepositorInsertMonoamine oxidase type A (MAOA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-488PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
Plasmid#134859PurposeExpresses EGFP-tagged ERGIC markerDepositorInsertERGIC53 (Lman1 Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)ExpressionMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro
Plasmid#211468PurposeLentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminalDepositorInsert4xGFP11-SEC61B
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TevC-IRES-SP6-Cre
Plasmid#171620PurposeM13-TevC with CreDepositorInsertM13-TevC-IRES-SP6-Cre
UseAAV and Synthetic BiologyTags6x HisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BCR-ABL1-p190-IRES-EGFP
Plasmid#219964PurposeExpress human BCR-ABL1-P190 in mammalian cellsDepositorInsertBCR-ABL1-P190 (ABL1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
Plasmid#129717PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLSDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherry-weak NLSExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin
Plasmid#134662PurposeExpresses EGFP-tagged plasma membrane markerDepositorInsertvesicle-associated membrane protein 5 (VAMP5 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394
Plasmid#128386PurposeExpresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsC-terminal luc2 fragment and N-terminal luc2 frag…ExpressionMammalianMutationN terminal carbohydrate recognition domain fused …PromoterEF1A and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
tetO-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69545PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.DepositorInserteGFP
UseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER
Plasmid#113898PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligaseDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)
Plasmid#132926PurposepMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertLuc2P
UseLuciferase and Synthetic Biology; Pmvp gateway en…TagsPEST destabilization domainExpressionMutationPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro GFP-3xFLAG
Plasmid#201636PurposeOver-expression of 3xFLAG-tagged GFPDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP
Plasmid#113888PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HEXIM1
Plasmid#200726PurposeLentiviral expression of Flag-Tagged human HEXIM1DepositorInsertHEXIM1 (HEXIM1 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50455PurposeDouble floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin
Plasmid#134857PurposeExpresses EGFP-tagged synaptic vesicle markerDepositorInsertRas-related protein 3C
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
Plasmid#145843PurposeLentiviral vector genome to ectopically express human TMPRSS2 variant 1DepositorInserthuman TMPRSS2 variant 1 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationG8VPromoterSFFVAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1365 LV EF1a-CD28 IRES-EGFP
Plasmid#201921Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD28DepositorInsertCD28 (CD28 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-TfR20-SNAP-IRES-Puro
Plasmid#171018PurposeLentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cellsDepositorInsertTransferrin receptor (Human) - SNAP-tag (TFRC Human)
UseLentiviralTagsSNAPtag and TfRExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Calr-KDEL-IRES-Puromycin
Plasmid#134664PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcalreticulin (CALR Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 18-413PromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
Plasmid#209410PurposeExpression of wild-type neonatal isoform of human SCN2A.DepositorInsertSCN2A Variant 4, stabilized (SCN2A Human)
UseTagsExpressionMammalianMutationIVS intron inserted after bp 4551 of the SCN2A OR…PromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPA1
Plasmid#200727PurposeLentiviral expression of Flag-Tagged human HNRNPA1DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
Plasmid#32520DepositorInsertConstitutively active Rheb (S16H) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationS16H to make constitutively activePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX
Plasmid#208696PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164
Plasmid#122966PurposeExpresses 3XFlag-tagged human CEP164 in mammalian cellsDepositorInsertCEP164 (CEP164 Human)
UseLentiviralTags3XFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)
Plasmid#203907PurposeEncodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cellsDepositorInsertGal4LOV(SK22)-VP64-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204340PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-s/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
UseTagsExpressionMammalianMutationT27stopPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV CAMKII PSAM4 GlyR IRES EGFP
Plasmid#119744PurposeChemogenetic inhibitor expression plasmidDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromoterCamKIIAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
Plasmid#126552PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsCD8a and GFPExpressionMutationDelta gag/polPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NGN2-IRES-GFP-T2A-Sox11
Plasmid#90214PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX
Plasmid#208693PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-PKIalpha-IRES-nls-mRuby2
Plasmid#63059PurposeExpresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertPKIalpha (Pkia Mouse, Synthetic)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT K78E-IRES-Puro
Plasmid#182591Purposeexpresses TERT K78E in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationK78EPromoterEF-1aAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP
Plasmid#113884PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterpHR-CMV-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
Plasmid#105922PurposeAAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITRDepositorInsertsmKate2
IRES-Multiple cloning site
UseAAVTagsExpressionMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-Flag-CDK19-W105M-IRES-ZsGreen
Plasmid#68859PurposeExpresses Human CDK19 W105M MutantDepositorInsertCyclin-Dependent Kinase 19 (CDK19 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA
Plasmid#178518PurposeFor making Rabies virus SAD B19 strain L cell line with hyPBaseDepositorInsertB19L-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX
Plasmid#208697PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
Plasmid#134308PurposeExpresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)DepositorInsertsUseTagsTdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1a-MICB*005-IRES-ZsGreen
Plasmid#114008PurposeInduces expression of human MICB allele 005 cDNADepositorInsertMICB*005 (MICB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Membrin-IRES-Puromycin
Plasmid#134865PurposeExpresses EGFP-tagged Golgi apparatus markerDepositorInsertMembrin (GOSR2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin
Plasmid#134864PurposeExpresses EGFP-tagged nuclear envelope markerDepositorInsertemerin (EMD Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only