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173,387 results

Showing: 11701 - 11730 of 173387 results

  3. GuideVector_BbsI

    Plasmid
    #110692
    Purpose
    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.
    Depositor
    Douglas Mahoney
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110692-plasmid-map-sequence-id-211319

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  5. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  13. pGAPTrap-eGFP-IRESMuro

    Plasmid
    #82504
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82504-plasmid-map-sequence-id-156573

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  16. pRosa26-GT

    Plasmid
    #40025
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    beta-globin intron
    Neo
    tdT-3Myc
    diphteria toxin A
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available Since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    40025-plasmid-map-sequence-id-210933

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  26. AAVS1-iPE-Plus

    Plasmid
    #214062
    Purpose
    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Article
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available Since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214062-plasmid-map-sequence-id-429371

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  28. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129593-plasmid-map-sequence-id-254271

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Showing: 11701 - 11730 of 173387 results