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We narrowed to 128,558 results for: CIL

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Showing: 781 - 800 of 128558 results

  1. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106280-plasmid-map-sequence-id-512176

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  6. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61358-plasmid-map-sequence-id-99789

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  14. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid
    #145780
    Purpose
    Over expression of SARS-CoV-2 S (spike) in mammalian cell line
    Depositor
    Zhaohui Qian
    Publication
    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (S SARS CoV 2)
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Codon optimized and C-terminal 19 amino acids del…
    Promoter
    CMV
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145780-plasmid-map-sequence-id-354441

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  17. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, AAV Retrograde, and AAV rh10
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105530-plasmid-map-sequence-id-454420

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Showing: 781 - 800 of 128558 results