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  7. p426_Cas9_gRNA-ARS208a

    Plasmid
    #87383
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87383-plasmid-map-sequence-id-193425

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  11. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Use
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

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  12. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

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  20. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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