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pDEST-CMV mCherry-GFP-LC3B WT

Plasmid

#123230

Purpose

Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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C1(1-29)-TurboID-V5_pLX304

Plasmid

#107175

Purpose

expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Lentiviral

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSB700

Plasmid

#64046

Purpose

Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

Depositor

George Church

Publication

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6, CAGGS

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Publication

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pCAG-Brainbow3.0

Plasmid

#45176

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCAG-Brainbow3.0

Expression

Mammalian

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pENTR4-GFP-C1 (w392-1)

Plasmid

#17396

Purpose

Gateway entry vector for N-term eGFP fusion, frame C1

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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GFP-SMARCA4

Plasmid

#65391

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SMARCA4 (SMARCA4 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCFD6

Plasmid

#73915

Purpose

tissue-specific multiplex gRNA plasmid

Depositor

Simon Bullock

Publication

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

:tRNA:gRNA:tRNA:gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

UAS-DSCP

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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pGFP-Rab8A

Plasmid

#24898

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hRab8A

Tags

GFP

Expression

Mammalian

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pLS-SceI

Plasmid

#137725

Purpose

Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

Depositor

Nadav Ahituv

Publication

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Type

Empty backbone

Use

Lentiviral

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE_puro

Plasmid

#118692

Purpose

Empty vector to express overexpression cDNA

Depositor

Christopher Vakoc

Publication

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

Plasmid

#101178

Purpose

Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/H698A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGBKT7-GW

Plasmid

#61703

Purpose

Gateway compatible Y2H vector - BD

Depositor

Yuhai Cui

Publication

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pMXs-gw

Plasmid

#18656

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Gateway cassette A

Use

Retroviral

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Promoter

35S

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSLIK-Venus

Plasmid

#25734

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Flag-HA-UCHL1

Plasmid

#22563

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL1 (UCHL1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Publication

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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