167,910 results

1. pMT85_SpdCas9_pmcDA1

   Plasmid

   #204614

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpRYc

   Plasmid

   #175575

   Purpose

   Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

   Insert

   SpRYc
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   PID of SpRY grafted onto the N-terminal domain of…

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 Flag Jnk2a2

   Plasmid

   #13755

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2a2 (MAPK9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

   Plasmid

   #195547

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5F Smad3

   Plasmid

   #12625

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

   Plasmid

   #127660

   Purpose

   Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Mutation

   E225A/D227A

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_HNF4A_P2A_Hygro_Barcode

   Plasmid

   #120450

   Purpose

   Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SCTR-Tango

   Plasmid

   #66501

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   SCTR (SCTR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. AICSDP-63: ACTN2-mEGFP

   Plasmid

   #124607

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2314

    Plasmid

    #142029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RXRB (RXRB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p006-GFP-pBAD

    Plasmid

    #108315

    Purpose

    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    GFP under control of arabinose-inducible promoter pBAD
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

    Plasmid

    #55220

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6 and MBP, Asn10

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. DL146_plenti_TRE_GFP-iPAK4

    Plasmid

    #187446

    Purpose

    Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    INKA-box-Pak4
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD57

    Plasmid

    #119882

    Purpose

    Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_GFP+
    

    Expression

    Bacterial

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPOTv7-blast-mStayGold[E138D]

    Plasmid

    #221049

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mStayGold[E138D]
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-Rab8a-wt

    Plasmid

    #86078

    Purpose

    A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Src-Y530F

    Plasmid

    #124659

    Purpose

    Human c-Src with Y530F mutation

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    cSrc (SRC Human)
    

    Expression

    Mammalian

    Mutation

    Y530F

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3173

    Plasmid

    #144649

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-7 asyn S129E

    Plasmid

    #36050

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129E (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S129E

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSF-1b-S2

    Plasmid

    #128590

    Purpose

    Expresses tagless S2 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S2 (rpsB E.coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. DL033:cFos::eGFP-iPAK4

    Plasmid

    #177883

    Purpose

    eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    cFos

    Available Since

    July 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. AICSDP-34: TNNI1-mEGFP

    Plasmid

    #114411

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pNESx2-EGFP-C1-PABDx2-Spo20

    Plasmid

    #220082

    Purpose

    PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Tags

    X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

    Expression

    Mammalian

    Mutation

    amino acids 51-91, tandem dimer

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. Mito-SNAP

    Plasmid

    #206480

    Purpose

    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    

    Tags

    SNAPf

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. AAVS1-Puro CAG CLTX-CAR

    Plasmid

    #157743

    Purpose

    knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDD108

    Plasmid

    #119884

    Purpose

    Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    BbaJ23104_EYFP
    

    Expression

    Bacterial

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. His-GFP-Rim2

    Plasmid

    #170869

    Purpose

    Mammalian expression of His-GFP-Rim2.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV3Tag8li-hDDX41-HA

    Plasmid

    #165469

    Purpose

    Human DDX41 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    DEAD-box helicase 41 (DDX41 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNB22

    Plasmid

    #103059

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    hmpAp

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. DT041A

    Plasmid

    #159402

    Purpose

    Constitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. iF58 PB-Zim3-dCas9-mScarlet-Puro

    Plasmid

    #204728

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pD454-SR rat Alpha-synuclein

    Plasmid

    #89074

    Purpose

    bacterial expression of rat Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Rat)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pcDNA3-eGFP-NLS-RNF168

    Plasmid

    #133977

    Purpose

    mammalian expression vector of eGFP tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid

    #134925

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT1UciMP

    Plasmid

    #62215

    Purpose

    UAS transgenesis vector

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Promoter

    UAS-E1b

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid

    #134924

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge