161,670 results
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pcDNA-Flag-Trib3
Plasmid#131157PurposeMammalian expression of flag-tagged mouse TRIB3DepositorInsertTrib3 (Trib3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2
Plasmid#67625PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switchDepositorInserthBACCS2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Centrin2-N-10
Plasmid#55018PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertCentrin2 (CETN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR1-EYFP
Plasmid#114370Purposeexpresses Flpo recombinase-dependent GtACR1DepositorInsertGtACR1-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK511
Plasmid#114686PurposeExpress Mem-BFP-iLIDDepositorInsertMem-BFP-iLID
UseTagsExpressionMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso2
Plasmid#159586PurposeExpress N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQL47 EGFP-VAMP-GST
Plasmid#122971PurposeReporter of botulinum toxin B cleavageDepositorInsertEGFP-VAMP3-GST
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-12x
Plasmid#61699PurposeExpresses PTDH mutant Opt12xDepositorInsertOpt12x
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mito-TurboID
Plasmid#200960PurposeTransiently expresses mitochondria localized TurboID with V5 tagsDepositorInsertmito-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro
Plasmid#227253PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
Plasmid#188899PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tubulin-mGreenLantern
Plasmid#164460PurposeLocalization of mGreenLantern fluorescent protein to tubulinDepositorInsertmGreenLantern
UseTagsTubulinExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-PDI
Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MmACE2 (Mouse)
Plasmid#158087PurposeExpresses mouse ACE2 in mammalian cellsDepositorInsertMouse ACE2 (Ace2 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS
Plasmid#145268PurposeMammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLSDepositorInsertRUNX2(+10A)-IDR (RUNX2 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRRP6_Z
Plasmid#148131PurposeMammalian Expression of HsRRP6DepositorInsertHsRRP6 (EXOSC10 Human)
UseTagsExpressionMammalianMutation2 silent mutations compared to the sequence of is…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-dDIO hChR2(H134R)-EYFP
Plasmid#55640PurposeDre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only
