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Showing: 2251 - 2300 of 4641 results

  17. pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP

    Plasmid
    #226113
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-1-5p Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226113-plasmid-map-sequence-id-448319

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  18. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid
    #226114
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226114-plasmid-map-sequence-id-448323

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  19. pEJS1352 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-22 CMV-BFP

    Plasmid
    #226111
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-22 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226111-plasmid-map-sequence-id-448317

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  23. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid
    #169135
    Purpose
    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Coh7
    Use
    Tags
    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169135-plasmid-map-sequence-id-361163

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  30. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  33. iDianiSnFR

    Plasmid
    #177741
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iDianiSnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177741-plasmid-map-sequence-id-348683

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  34. iCytSnFR

    Plasmid
    #177738
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCytSnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177738-plasmid-map-sequence-id-348679

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  35. iCyt_BrEt_SnFR

    Plasmid
    #177740
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_BrEt_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    177740-plasmid-map-sequence-id-349387

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  36. iCyt_F_SnFR

    Plasmid
    #177739
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_F_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177739-plasmid-map-sequence-id-348682

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  37. NSS-GFP

    Plasmid
    #163661
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TNF…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163661-plasmid-map-sequence-id-322793

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Showing: 2251 - 2300 of 4641 results