167,729 results

1. CBh-dCas9-Mll3SET

   Plasmid

   #108452

   Purpose

   Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

   Depositor

   Bing Ren

   Article

   Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

   Insert

   dCas9, MLL3 (Kmt2c Mouse)
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-EGFP-ALDH1A1-Neo

   Plasmid

   #189742

   Purpose

   The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A1 (ALDH1A1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-BB

   Plasmid

   #60521

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma8-GFP2

   Plasmid

   #140990

   Purpose

   Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma8-GFP2 (GNG8 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

   Plasmid

   #154840

   Purpose

   Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   HYGR∆ encodes aa 1-226

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. IP3 receptor, type 1 scFv [L24/21]

   Plasmid

   #206790

   Purpose

   Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/21 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_human CDK1

   Plasmid

   #118597

   Purpose

   One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   guide RNA against human CDK1
   

   Use

   CRISPR

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiDP1.0

   Plasmid

   #171094

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2060

    Plasmid

    #141899

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1X-Nup88

    Plasmid

    #87338

    Purpose

    To express human Nup88 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP88 (NUP88 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p413 TEF NAPstarC

    Plasmid

    #232737

    Purpose

    Expresses control sensor NAPstarC in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstarC
    

    Expression

    Yeast

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. (133) pcDNA3.1-Nup62-Flag-EPEA

    Plasmid

    #160744

    Purpose

    Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    Nup62-Flag-EPEA
    

    Tags

    EPEA and Flag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid

    #229677

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    SNAP, 3xV5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-rtTA3G

    Plasmid

    #139556

    Purpose

    rtTA ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    rtTA3G
    

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pvimentin-emiRFP703

    Plasmid

    #136566

    Purpose

    Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    vimentin-emiRFP703
    

    Tags

    emiRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCRISPRa-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/gene

    Pooled Library

    #83999

    Purpose

    Mouse CRISPRa Pooled Library targeting cancer and apoptosis genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pET Flag TEV LIC cloning vector (1L)

    Plasmid

    #29662

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    Flag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221_SLC15A4

    Plasmid

    #131910

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC15A4 (SLC15A4 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pMRE-Tn7-132

    Plasmid

    #118550

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMRE-Tn7-135

    Plasmid

    #118553

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. TFORF2895

    Plasmid

    #144355

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MTA1 (MTA1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHR-Flag-APH4-Fibcon-icam

    Plasmid

    #233077

    Purpose

    Expresses synCAM-APH4-Fibcon-icam

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    APH4-FIBCON-ICAM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCR3.1-OPNa

    Plasmid

    #107980

    Purpose

    expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    full-length osteopontin (SPP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSG5-His-SUMO1

    Plasmid

    #17271

    Depositor

    Stephen Goff

    Article

    Yueh et al J Virol. 2006 Jan . 80(1):342-52.

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3-SEP-Glur2 (MCA10)

    Plasmid

    #64941

    Purpose

    mammalian expression of rat GluR2

    Depositor

    Jeremy Henley

    Article

    Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

    Insert

    GluR2 (Gria2 Rat)
    

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV-syn-jYCaMP1s (AAV1)

    Viral Prep

    #135424-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-jYCaMP1s (#135424). In addition to the viral particles, you will also receive purified pAAV-syn-jYCaMP1s plasmid DNA. Synapsin-driven expression of calcium sensor YCaMP1s. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Promoter

    Syn

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

30. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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