Addgene

We narrowed to 19,618 results for: REN;

Showing: 2351 - 2400 of 19618 results

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pJUMP18-B0034-MV_RN

Plasmid

#127017

Purpose

Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m-MV_RN

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBS cErm (CT#1054)

Plasmid

#13907

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Erm (ETV5 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pET29NK-LGK/mGFPmut3

Plasmid

#81168

Purpose

GFP fusion to levoglucosan kinase

Depositor

Timothy Whitehead

Article

Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

Insert

Levoglucosan kinase wild-type

Use

Tags

His6x and mGFPmut3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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hPlekhm1_pIRES puro Glue

Plasmid

#73453

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
HOXC6-pCT

Plasmid

#21000

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC6 (HOXC6 Human)

Use

Tags

Expression

Mammalian

Mutation

Long 5' UTR, approx 480 nt

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Cas-Acr (D14A/G38A)

Plasmid

#128118

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (D14A/G38A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBS cCollagen XII (CT#1051)

Plasmid

#13916

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Collagen XII (COL12A1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-FL

Plasmid

#127795

Purpose

Expresses full-length ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-mCitrine

Plasmid

#117989

Purpose

Chloroplast-targeted expression of mCitrine controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-mCitrine

Use

Plant/bacteria binary vector

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0032-MV_RN

Plasmid

#127015

Purpose

Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m-MV_RN

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-min2

Plasmid

#126956

Purpose

"Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS cFgfr2 (CT#221)

Plasmid

#14003

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Fgfr2 (FGFR2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
HA PRAS40 P185A

Plasmid

#15675

Depositor

John Lawrence

Article

Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

Insert

PRAS40 P185A (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P185A

Promoter

Available since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Nup88 (551-741)

Plasmid

#64252

Purpose

Mammalian expression of C-terminal domain of Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

C-terminal domain (551-741)

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pBS cPea3 (CT#1024)

Plasmid

#13906

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (ETV4 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Use

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDLterm

Plasmid

#211903

Purpose

Base plasmid for dual-luciferase constructs to measure terminator strength

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

35S enhancer + 35S minimal promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-9myc

Plasmid

#212787

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP

Plasmid

#212768

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-yeGFP

Plasmid

#212766

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-yeGFP

Plasmid

#212767

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo-yeGFP

Plasmid

#212771

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K48R)-yeGFP

Plasmid

#212772

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K63R)-yeGFP

Plasmid

#212773

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-His6

Plasmid

#212776

Purpose

C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-His6

Plasmid

#212777

Purpose

C-terminal PCR-based tagging with CUbo-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-His6

Plasmid

#212778

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-His6

Plasmid

#212779

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-yeGFP-His6

Plasmid

#212782

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-9myc

Plasmid

#212786

Purpose

C-terminal PCR-based tagging with CUbo-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-9myc

Plasmid

#212788

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-AID*-GFP

Plasmid

#212770

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K63R)

Plasmid

#212764

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K48R)

Plasmid

#212763

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo

Plasmid

#212762

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-His6-CUbo-MCS

Plasmid

#212725

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-His7-NUbo-MCS

Plasmid

#212724

Purpose

Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-NUbo-MCS-His6

Plasmid

#212723

Purpose

Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-NUbo-E3(63)-His6

Plasmid

#212717

Purpose

Bacterial expression of NUbo-E3(63)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Tags

6xHis and NUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-NUbo-E3(48)-His6

Plasmid

#212714

Purpose

Bacterial expression of NUbo-E3(48)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Tags

6xHis and NUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FB395

Plasmid

#203631

Purpose

TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1aG2b.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB396

Plasmid

#203632

Purpose

TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1ab.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB397

Plasmid

#203633

Purpose

TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1abc.3:mPAF:Luc:TtrpC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB2880

Plasmid

#193117

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3277

Plasmid

#193131

Purpose

A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1abc.4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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