161,492 results

1. pLKO-RB1-shRNA63

   Plasmid

   #25641

   Purpose

   3rd generation lentiviral vector

   Depositor

   Todd Waldman

   Article

   Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

   Insert

   RB1-shRNA 63 (RB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-Blast-PIP-FUCCI

   Plasmid

   #138715

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX307 Luciferase

   Plasmid

   #117734

   Purpose

   Open reading frame vector encoding Luciferase

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   firefly luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA-3xHA-hTERT

   Plasmid

   #51637

   Purpose

   Mammalian expression of 3xHA tagged human TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   TERT (TERT Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV1)

   Viral Prep

   #100833-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Tags

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

   Plasmid

   #83842

   Purpose

   Fluorescent probe for S/G2 cell cycle transition and G2/M transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. PIEZO1-HaloTag

   Plasmid

   #207834

   Purpose

   PIEZO1 fusion for biochemistry and live imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   PIEZO1 (PIEZO1 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CMV-jGCaMP8f

   Plasmid

   #162373

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MORF library without GFP and mCherry controls

    Pooled Library

    #192821

    Purpose

    Barcoded transcription factor ORF library for high throughput screening

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA-HDAC6-FLAG

    Plasmid

    #30482

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK381 (AAVS1 CMV-OsTIR1F74G)

    Plasmid

    #140536

    Purpose

    AAVS1 CMV-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28b-RfxCas13d-His

    Plasmid

    #141322

    Purpose

    Plasmid for bacterial expression and purification of RfxCas13d protein

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1-TFEB

    Plasmid

    #38119

    Purpose

    Mammalian expression of Transcription factor EB fused to EGFP

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMAL-CellTag-V1

    Pooled Library

    #115643

    Purpose

    Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

    Depositor

    Samantha Morris

    Article

    Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

    Has Service

    Concentrated Lentiviral Prep

    Expression

    Mammalian

    Species

    Synthetic

    Use

    Lentiviral

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pDY1656 STITCHR Gluc AAVS1 payload

    Plasmid

    #234827

    Purpose

    Gluc exon 2 with 50bp AAVS1 homology arms

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    Gluc exon 2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid

    #85181

    Purpose

    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

    Depositor

    Anjana Rao

    Article

    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

    Insert

    CA-RIT-NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTM-BPL

    Plasmid

    #173766

    Purpose

    Fluorescent labeling of the nuclear envelope

    Depositor

    Shinji Sueda

    Article

    Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

    Insert

    PDGF receptor TM domain, Biotin protein ligase
    

    Use

    Tags

    FLAG and Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBCCP-GFP-NLS

    Plasmid

    #173923

    Purpose

    Fluorescent labeling of the nuclear envelope

    Depositor

    Shinji Sueda

    Article

    Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

    Insert

    Biotin carboxyl carrier protein, AcGFP1
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PCS2-FlipGFP(Casp3 cleavage seq)

    Plasmid

    #124432

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq)
    

    Use

    Zebrafish (good expression)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Laconic/pcDNA3.1(-)

    Plasmid

    #44238

    Purpose

    a genetically-encoded sensor for lactate concentration, transport and metabolic flux

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

    Insert

    Laconic (LACtate Optical Nano Indicator from CECs)
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid

    #208717

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Ma-PylRS-lib1

    Pooled Library

    #224585

    Purpose

    Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Expression

    Bacterial

    Species

    Methanomethylophilus alvus

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

28. pDAC565

    Plasmid

    #195242

    Purpose

    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-hLC3B-pcDNA3.1

    Plasmid

    #40827

    Depositor

    David Rubinsztein

    Article

    Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    NF-kappaB binding site

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cbh

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)

    Viral Prep

    #131004-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Tags

    None

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

36. HA-APP-mGFP

    Plasmid

    #196696

    Purpose

    Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    HA and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK5-HA-Ubiquitin-K63

    Plasmid

    #17606

    Purpose

    Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K63 only, other lysines mutated to arginines. Enh…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ABE8e-SpRY

    Plasmid

    #226858

    Purpose

    Expresses ABE8e-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge