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Plasmid#194314PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertNGFR-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro EGFP cLAP -1
Plasmid#194312PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertcLap(EGFP)-P2A-Hygro
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_SulR(CDS)
Plasmid#186423Purposeprovides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)DepositorInsertSulR
UseSynthetic Biology; Cds (part b3-b4-b5), goldenbra…TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP129
Plasmid#189537PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-GFP-rG1
Plasmid#188967PurposeIPTG inducible GFP with sgRNADepositorInsertsGFP
sgRNA: agtggaaaacaatgcgaccgactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-GFP-rG2
Plasmid#188968PurposeIPTG inducible GFP with sgRNADepositorInsertsGFP
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3×FLAG-SLC35A2
Plasmid#186283Purposetransient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorInsertSLC35A2 (SLC35A2 Human)
UseTags3×FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1023-Tier1-PhCMV-p2A-PuroR
Plasmid#169497PurposeTier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).DepositorInsertPCMV-driven P2A-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1021-Tier1-PhCMV-PuroR
Plasmid#169492PurposeTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)DepositorInsertPCMV-driven puromycin N-acetyl-transferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5b_Ptet
Plasmid#179338PurposegRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC0x_gRNA_Pos5a_Ptet
Plasmid#179337PurposegRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-eGFP-TAX1BP1 Y635A
Plasmid#175772PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationY635APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 N637A
Plasmid#175773PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationN637APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_APV-SBP-Dendra2-ER-5
Plasmid#170679PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.DepositorInsertsAPV-SBP-Dendra2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term SMT3 NikJC199U
Plasmid#174210PurposeExpression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term SMT3 NikJC199U
Plasmid#174362PurposeExpression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP NikJC199U
Plasmid#174364PurposeExpression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNArodA
Plasmid#149658Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJo55-CA1
Plasmid#159624PurposePlasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.DepositorInsertC. annuum eIF4E-1
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC1-1-170
Plasmid#173467PurposePGRMC1 lacking the extreme C-terminusDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsFLAGExpressionMammalianMutationDeleted for amino acids 171-194PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-1-54
Plasmid#173472PurposePGRMC1 lacking the C-terminal region downstream of the transmembrane domainDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 55-194PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-21-195
Plasmid#173473PurposePGRMC1 lacking the N-terminal region upstream of the transmembrane regionDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 1-20PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-OPTNS177DS473D
Plasmid#171425PurposeFor the purifcation of phosphorylation mimic proteinDepositorInsertOPTN (OPTN Human)
UseTagsExpressionBacterialMutationphosphorylation mutant (S177D, S473D)PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs- FIP200 delta641-779-MBP
Plasmid#171411PurposeFor the purifcation of human ULK1 truncated complexDepositorInsertFIP200 (RB1CC1 Synthetic)
UseTagsExpressionMammalianMutationtruncation (delta641-779-MBP)PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9H(-10TC)_arr2
Plasmid#149587Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAflaB
Plasmid#149561PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)
Plasmid#159888PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-1 (w ATG)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)
Plasmid#159889PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-2(no_start)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PflaBS-sgRNAflaB
Plasmid#149643Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PflaBSAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_arr2
Plasmid#149582Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0826L-sgRNAflaB
Plasmid#149648Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0826LAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNArodA
Plasmid#149655Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTS-sgRNAflaB
Plasmid#149644Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PresTSAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTL-sgRNAflaB
Plasmid#149645Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PresTLAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0026-sgRNAflaB
Plasmid#149646Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0026Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only