167,948 results

1. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Carol Gross, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ISceI-GR-RFP

   Plasmid

   #17654

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   C655G mutation in the glucocorticoid receptor (se…

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUW-tetO-3xFLAG-GATA2

   Plasmid

   #125600

   Purpose

   doxycycline-inducible expression of human GATA2 (3xFLAG tagged) in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

   Insert

   GATA binding protein 2 (GATA2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG tag

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCENPTΔC-dCas9-3xGFP

   Plasmid

   #198325

   Purpose

   Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   CENPTΔC (CENPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP x 3 and dCas9

   Expression

   Mammalian

   Mutation

   truncation - encodes only amino acids 1-375

   Promoter

   CMV-TetO

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPHK2 gRNA (BRDN0001146242)

   Plasmid

   #77095

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. UbcH5c WT pET28a

   Plasmid

   #12643

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Expression

   Bacterial

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPT24-mtnluc

   Plasmid

   #184339

   Purpose

   Transformation of mitochondrial DNA in yeast

   Depositor

   Martin Ott

   Article

   Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

   Insert

   nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
   

   Expression

   Yeast

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL003

   Plasmid

   #215335

   Purpose

   amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

   Depositor

   Gene-Wei Li

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pSpank

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAXEF-Rap1-WT

    Plasmid

    #32697

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Promoter

    EF promoter

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pLVX-Tight-Puro-KrasG12D2B

    Plasmid

    #139786

    Purpose

    Lentiviral tet-inducible expression of KrasG12D (mouse)

    Depositor

    Joan Massague

    Article

    Su et al Nature. 2020 Jan;577(7791):566-571. doi:

    Insert

    KrasG12D (Kras Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G12D

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXL-BacII-CAG-mCherry

    Plasmid

    #170519

    Purpose

    Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

    Insert

    CAG-mCherry
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPdR

    Plasmid

    #85083

    Purpose

    Expresses putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lamin A-mRFP

    Plasmid

    #124268

    Purpose

    Wild-type Lamin A, C-terminally tagged with mRFP

    Depositor

    Eric Schirmer

    Insert

    LMNA (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7-p73DD-pcDNA3

    Plasmid

    #22978

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p73 (TP73 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 327-…

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-MCP-dNS-SRRM1-V5-mCherry

    Plasmid

    #235095

    Purpose

    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    

    Expression

    Mammalian

    Mutation

    The SRRM1 entry clone from DNASU was modified usi…

    Promoter

    CAG

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-GFP-CD63

    Plasmid

    #194989

    Purpose

    overexpress CD63

    Depositor

    Angelique Bordey

    Insert

    GFP-CD63
    

    Expression

    Bacterial

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCap-SG5-Apr

    Plasmid

    #227579

    Purpose

    Capture plasmid with apramycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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