We narrowed to 72,188 results for: LAS

1. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

   Plasmid

   #197687

   Purpose

   plasmid for expressing the DNMT3bCD

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD hCPS1 AD

   Plasmid

   #188130

   Purpose

   Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

   Plasmid

   #166413

   Purpose

   express hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   Protection Of Telomeres 1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   ΔOB deleted 125 a.a

   Promoter

   ef1-a

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MDH1-miR-31SM-PGK-GFP-Blast

   Plasmid

   #31516

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-31 (Mir31 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   seed mutant control

   Promoter

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pMXS-IRES-BLAST flag-CAD E1368A

   Plasmid

   #188141

   Purpose

   Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

   Plasmid

   #205541

   Purpose

   Express EPN1-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

   Plasmid

   #205545

   Purpose

   Express EPN11-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-BLAST flag-CAD S1396A

   Plasmid

   #188126

   Purpose

   Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR

    Plasmid

    #48157

    Purpose

    Transgenically expresses firefly luciferase protein in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    luciferase
    

    Use

    Luciferase; T. cruzi expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLENTI CMV GFP-TREX1 V235fs BLAST

    Plasmid

    #164236

    Purpose

    lentiviral plasmid for GFP-TREX1 V235fs

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V235 frame shift mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLK89 - Edit-directing plasmid cloning insert

    Plasmid

    #98846

    Purpose

    Contains the insert used for step 2 of edit-directed plasmid cloning

    Depositor

    Leonid Kruglyak

    Insert

    KanR
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Changed sequence to remove a MluI cut site

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

    Plasmid

    #71219

    Purpose

    dCas9 fused to the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

    Plasmid

    #192239

    Purpose

    knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

    Plasmid

    #192242

    Purpose

    knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

    Plasmid

    #192241

    Purpose

    knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pShuttle-EGFP-CLASP2 340-1362 9xS/A

    Plasmid

    #24373

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1362) 9xS/A (CLASP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MDH1-miR-222-PGK-GFP-Blast

    Plasmid

    #31522

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-222 (Mir222 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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