We narrowed to 11,299 results for: 221

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pDONR221-EWSR1 g1750a No Stop

Plasmid

#84894

Purpose

DONOR vector for Gateway cloning of EWSR1 g1750a No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

g1750a No Stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 g1750a Stop

Plasmid

#84893

Purpose

DONOR vector for Gateway cloning of EWSR1 g1750a Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

g1750a

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 c1655t Stop

Plasmid

#84891

Purpose

DONOR vector for Gateway cloning of EWSR1 c1655t Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

c1655t

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-G1238A-C1240T

Plasmid

#24739

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Mutation

G replaced with A at position 1238 and C replaced…

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

Plasmid

#186987

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, Gluc crRNA guide

Use

AAV

Mutation

s1006-gggs-d1221 deletion

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

Plasmid

#195101

Purpose

Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

CTCF (CTCF Human)

Tags

AID, HA, and eGFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA

Plasmid

#108727

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA

Plasmid

#108725

Purpose

Expresses N-terminus to aa251 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa251

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1543 - pAAV-AiE2210m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243057

Purpose

AiE2210m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific population sof brain cells.

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Insert

SYFP2

Use

AAV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

Plasmid

#242666

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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VV221: sdChR(C138S)-TS-eGFP-ER in fck

Plasmid

#58513

Purpose

Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-eGFP-ER

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R(1-300)

Plasmid

#79029

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5-21R (1-300)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Plasmid

#159866

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Plasmid

#159864

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

Plasmid

#240517

Purpose

Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

Depositor

Erik Sontheimer

Article

Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

Insert

AcrIIC3

Tags

FLAG, hsa-miR-221 Target Sites, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-221-PGK-GFP-Blast

Plasmid

#31519

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-221SM-PGK-GFP-Blast

Plasmid

#31520

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
RCASBP-Y DV

Plasmid

#11478

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RCASBP-Y NHA-EGFP

Plasmid

#11481

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Enhanced Green Fluoresecent Protein

Use

Retroviral

Tags

HA

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only
RCASBP-Y CHA

Plasmid

#11480

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RCASBP-Y NHA

Plasmid

#11479

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ2087

Plasmid

#92414

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

Insert

TEV protease

Tags

MBP (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

tac

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-PTEN

Plasmid

#13039

Depositor

Alonzo Ross

Article

Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

Insert

PTEN (PTEN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pTFUbiCre

Plasmid

#139670

Purpose

Expression cassette for Cre recombinase in T-DNA vector

Depositor

James Birchler

Article

Gaeta et al Chromosoma. 2013 Jun;122(3):221-32. doi:

Insert

Cre recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk1-mCherry

Plasmid

#54466

Purpose

CDK1-mCherry in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

Cdk1 mCherry

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS253

Plasmid

#114271

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Mutation

*please see depositor comment below

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS415

Plasmid

#114272

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Mutation

*please see depositor comment below

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Dlx2

Plasmid

#15537

Depositor

John Rubenstein

Article

Porteus et al Neuron. 1991 Aug . 7(2):221-9.

Insert

Dlx2 (Dlx2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pCEP4-tat

Plasmid

#22502

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

Transactivator of Transcription

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pHEF-VSVG

Plasmid

#22501

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

Vesicular Stomatitis Virus Glycoprotein

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
pNHP

Plasmid

#22500

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

gag, pol, tat, rev

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
CyclinB-GFP

Plasmid

#54465

Purpose

CyclinB-GFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

CyclinB

Tags

GFP

Expression

Mammalian

Mutation

K67E and I150T mutations compared to GenBank refe…

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only
-24lacZ

Plasmid

#25422

Depositor

David J. Goldhamer

Article

Chen et al Dev Dyn. 2001 Jul . 221(3):274-88.

Insert

Myo D 5' flanking sequence (MYOD1 Human)

Use

Reporter construct

Tags

lacZ

Expression

Mammalian

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
RCASBP-Y DV

Plasmid

#11478

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RCASBP-Y NHA-EGFP

Plasmid

#11481

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Enhanced Green Fluoresecent Protein

Use

Retroviral

Tags

HA

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only
RCASBP-Y CHA

Plasmid

#11480

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RCASBP-Y NHA

Plasmid

#11479

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES262 [prADH1-Msn2-mCherry, pnh604]

Plasmid

#103844

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pAdh1-Msn2-mCherry

Tags

mCherry

Expression

Yeast

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag

Plasmid

#240484

Purpose

Express engineered glycosylated eGFP in CHO cells

Depositor

Xi Ren

Article

Xing et al Acta Biomater. 2025 Nov;207:221-232. doi

Insert

glycosylated eGFP

Tags

3 repeats of hVEGF165 glycosylation sequon, 6xHis…

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-MBD2.5R (pc2395)

Plasmid

#211727

Purpose

Expresses MBD2 (aa153-221) RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.5 aa153-221

Tags

RFP

Expression

Insect

Promoter

Polyhedrin promotor

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-PTEN

Plasmid

#13039

Depositor

Alonzo Ross

Article

Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

Insert

PTEN (PTEN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk1-mCherry

Plasmid

#54466

Purpose

CDK1-mCherry in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

Cdk1 mCherry

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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