166,542 results

1. pCMV-PE6c

   Plasmid

   #207853

   Purpose

   Mammalian expression of PE6c prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6c
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRESTO-Tango Kit

   Plasmid Kit

   #1000000068

   Purpose

   Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.

   Depositor

   Bryan Roth

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pHR_PGK_antiCD19_synNotch_TetRVP64

   Plasmid

   #79126

   Purpose

   Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   antiCD19_synNotch_TetRVP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

   Plasmid

   #207507

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, HA-GD2-28z_CAR (NGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-evoCAST-TniQ

   Plasmid

   #234728

   Purpose

   Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseTniQ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-evoCAST-eCas8

   Plasmid

   #234727

   Purpose

   Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ePseCas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIRECT_22C

   Plasmid

   #91135

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-VPR_P2A_mCherry

   Plasmid

   #154193

   Purpose

   Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sp-dCas9-VPR_P2A_mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1as

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-hM3D(Gq)-mCherry

   Plasmid

   #50474

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cbh_v5 AAV-ABE C-terminal

    Plasmid

    #137178

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-mCherry (AAV8)

    Viral Prep

    #114472-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    mCherry

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cbh_v5 AAV-ABE N-terminal

    Plasmid

    #137177

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-ABE N-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-TEAD1

    Plasmid

    #229411

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-GFP

    Plasmid

    #11150

    Purpose

    Mammalian expression vector for expression of GFP (CAG promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EGFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. phL1A-pcDNA3

    Plasmid

    #12307

    Depositor

    Vance Lemmon

    Article

    Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry

    Plasmid

    #176016

    Purpose

    Mammalian expression of mCherry. Parton lab clone DFQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)

    Viral Prep

    #52925-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Baljit Khakh

    Article

    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

    Promoter

    Tags

    None

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only