We narrowed to 59,462 results for: plasmid
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Plasmid#50455PurposeDouble floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoterDepositorHas serviceAAV8InserthM4D(Gi)-IRES-mCitrine (CHRM4 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-EGFP (AAV PHP.eB)
Viral prep#50465-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE (AAV PHP.eB)
Viral prep#162375-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
ExpressionMammalianPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)
Viral prep#154867-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hIRE1a wt
Plasmid#20744DepositorPublicationAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPRIME-TET-GFP-FF3
Plasmid#11662Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorMutationPro2Ala, possibly Asp613Gly and changes made to A…Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available sinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CamKIIa-jGCaMP8f-WPRE (AAV1)
Viral prep#176750-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV Retrograde)
Viral prep#162374-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG-Hc∆18
Plasmid#84817PurposeMeasles virus hemagglutinin expressing plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMeasles virus H glycoprotein
ExpressionMammalianPromoterCMV IEAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFCcGi
Plasmid#59324PurposeInducible expression of GFP and constitutive expression of mCherryDepositorInsertsmCherry
EGFP
ExpressionBacterialPromoterPbad and rpsMAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSQT834
Plasmid#53371PurposeCsy4 and Cas9 nuclease expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy4-T2A-Cas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAKgfp1
Plasmid#14076DepositorInsertGreen fluorescent protein
ExpressionBacterialAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by