303,846 results

1. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

   Plasmid

   #246435

   Purpose

   Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.

   Depositor

   Ailong Ke

   Article

   Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

   Inserts

   O.gue IscB with dead mutations (D60A, H269A) and delta-TID
   EGFP
   

   Tags

   SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 60 to Alanine, changed Hist…

   Promoter

   CMV

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNP3440 [pNP2922 - ISCro4 bRNA (TBL2, WT + DBL1, WT) + ISCro4 Recombinase (WT)]

   Plasmid

   #247263

   Purpose

   Express ISCro4 recombinase and bridge RNA

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP2922 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNP3414 [pNP2922 - ISCro4 Enhanced TBL (TBL4, WT) + ISCro4 Recombinase (WT)]

   Plasmid

   #247261

   Purpose

   Express ISCro4 recombinase and bridge RNA

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP2922 - ISCro4 bRNA1-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL4)
   

   Expression

   Mammalian

   Mutation

   C60U, A61G, U73C, dU86, A87G, dU88

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

   Plasmid

   #247251

   Purpose

   Measure ISCro4 activity via luciferase expression

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. FEOX-PBCS-TBF2- MC2

   Plasmid

   #234406

   Purpose

   Tool for genetically encoded fluorescent biosensor of cellular iron microenvironment

   Depositor

   Carolyn Sangokoya

   Article

   Sangokoya et al Sci Rep. 2025 Oct 21;15(1):36596. doi: 1

   Inserts

   FEOX1 CONTROL
   FEOX
   

   Use

   Synthetic Biology

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   EF1-alpha and hpgk

   Available Since

   Oct. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-Myc-BirA

   Plasmid

   #234039

   Purpose

   Expresses Myc-His-BirA(R118G)

   Depositor

   Roderick O'Sullivan

   Article

   Garcia-Exposito et al Cell Rep. 2016 Nov 8;17(7):1858-1871. do

   Insert

   BirA
   

   Use

   Retroviral

   Tags

   Myc-6xHis

   Expression

   Mammalian

   Mutation

   R118G

   Available Since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 HIS-Ub-K48R

   Plasmid

   #221368

   Purpose

   Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

   Depositor

   Aga Gambus

   Article

   Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

   Insert

   Ubiquitin
   

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   Changed Lysine 48 to Arginine

   Promoter

   CMV

   Available Since

   Aug. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-Flag-SOD1-G93A

   Plasmid

   #235662

   Purpose

   Express human SOD1-G93A in mammalian cells

   Depositor

   Chen Seng Ng

   Article

   Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

   Insert

   SOD1-G93A (SOD1 Human)
   

   Tags

   Flag-tag at N-terminal

   Expression

   Mammalian

   Mutation

   changed Glycine 93 to alanine

   Promoter

   CMV promoter

   Available Since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBbsr2-RaichuEV-Rac1HRasCT

   Plasmid

   #214818

   Purpose

   Encoding a Förster resonance energy transfer (FRET) biosensor for Rac1 activity with cyan and yellow fluorescent protein.

   Depositor

   Michiyuki Matsuda

   Article

   Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

   Insert

   RaichuEV-Rac1HRasCT
   

   Tags

   HRasCAAX

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSIIbsr-Raichu-Rab5/PM

    Plasmid

    #214819

    Purpose

    Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    A Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
    

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPc3 N-RAS

    Plasmid

    #236450

    Purpose

    transient overexpression of N-RAS in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    N-RAS (NRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFPc1 H-RAS

    Plasmid

    #236449

    Purpose

    transient overexpression of H-RAS in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    H-RAS (HRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220731

    Purpose

    AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid

    #191212

    Purpose

    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    SARS-CoV-2 spike_WT (S SARS CoV-2)
    

    Tags

    HiBiT (high affinity small nanoluciferase fragmen…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid

    #191212

    Purpose

    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    SARS-CoV-2 spike_WT (S SARS CoV-2)
    

    Tags

    HiBiT (high affinity small nanoluciferase fragmen…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA FRT to BioID-cDIC1-3xFlag

    Plasmid

    #232417

    Purpose

    Expresses human dynein subunit DIC1 in mammalian cells.

    Depositor

    Samara Reck-Peterson

    Article

    Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

    Insert

    Dynein Intermediate Chain 1 (DYNC1I1 Human)
    

    Tags

    BioID and Flag-3x

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXTK024 [Origin_RSF1010_PartType1]

    Plasmid

    #229261

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    RSF1010 Origin
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ubi:ccdb + mcherry

    Plasmid

    #233303

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb + mcherry
    

    Use

    Gateway destination

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ubi:ccdb-3xHA + mcherry

    Plasmid

    #233302

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb-3xHA + mcherry
    

    Use

    Gateway destination

    Tags

    3xHA

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ubi:ccdb-eGFP + mcherry

    Plasmid

    #233300

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb-eGFP + mcherry
    

    Use

    Gateway destination

    Tags

    eGFP

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

    Plasmid

    #230360

    Purpose

    AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. sgPPP1R2

    Plasmid

    #227391

    Purpose

    Adhesion module, guide RNA recognizes PPP1R2

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    guide RNA targeting PPP1R2
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pACYC-yhaM-RFP

    Plasmid

    #207651

    Purpose

    Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    yhaM
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pUdOm7.1

    Plasmid

    #210287

    Purpose

    Empty backbone with zeocin resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMRS-dCas9

    Plasmid

    #220193

    Purpose

    mini-Tn7 dCas9 expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    promoterless

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGBW-m4046749

    Plasmid

    #145629

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. pGBW-m4046749

    Plasmid

    #145629

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCAG-iCre

    Plasmid

    #89573

    Purpose

    Expresses iCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iCre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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