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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,159 results for: ENA

Showing: 3301 - 3350 of 11159 results

  21. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Article
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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  23. pJG370

    Plasmid
    #91187
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    nAtCas9_D10A+gRNActrl+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91187-plasmid-map-sequence-id-182724

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  25. AA015

    Plasmid
    #216058
    Purpose
    Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    P2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216058-plasmid-map-sequence-id-431548

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  33. pSilencer2.1-U6-3JB1F+2

    Plasmid
    #190833
    Purpose
    For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190833-plasmid-map-sequence-id-379189

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  36. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid
    #185687
    Purpose
    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).
    Depositor
    Peter Walter
    Article
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Insert
    ERmembrane-GST-2xHaloTag-KKMP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185687-plasmid-map-sequence-id-366415

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  40. pJH4260

    Plasmid
    #191678
    Purpose
    Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    GCaMP6s
    Use
    Tags
    wScarlet
    Expression
    Worm
    Mutation
    Promoter
    Ptwk-40
    Available since
    Nov. 30, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    191678-plasmid-map-sequence-id-375288

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  45. Bongwoori

    Plasmid
    #63720
    Purpose
    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D
    Depositor
    Bradley Baker
    Article
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Insert
    Bongwoori
    Use
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. pSilencer2.1-U6-LRS1F50C

    Plasmid
    #190856
    Purpose
    For mammalian expression of LRS1F50C, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer is regulated by riboswitch elements.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    LRS1F50C (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190856-plasmid-map-sequence-id-379253

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Showing: 3301 - 3350 of 11159 results