We narrowed to 6,880 results for: GFP expression plasmids

1. 184_pAAV-ProD15-CatCh-GFP-WPRE

   Plasmid

   #125991

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD15

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 185_pAAV-ProD16-CatCh-GFP-WPRE

   Plasmid

   #125992

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD16

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 209_pAAV-ProD18-CatCh-GFP-WPRE

   Plasmid

   #125994

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD18

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 210_pAAV-ProD19-CatCh-GFP-WPRE

   Plasmid

   #125995

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD19

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 211_pAAV-ProD20-CatCh-GFP-WPRE

   Plasmid

   #125996

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD20

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 212_pAAV-ProD21-CatCh-GFP-WPRE

   Plasmid

   #125997

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD21

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 215_pAAV-ProD23-CatCh-GFP-WPRE

   Plasmid

   #125999

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD23

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 217_pAAV-ProD25-CatCh-GFP-WPRE

   Plasmid

   #126001

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProD25

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 155_pAAV-ProA4-CatCh-GFP-WPRE

   Plasmid

   #125888

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProA4

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 120_pAAV-ProC26-CatCh-GFP-WPRE

    Plasmid

    #125961

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC26

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 152_pAAV-ProC30-CatCh-GFP-WPRE

    Plasmid

    #125965

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC30

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 168_pAAV-ProC35-CatCh-GFP-WPRE

    Plasmid

    #125969

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC35

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 171_pAAV-ProC36-CatCh-GFP-WPRE

    Plasmid

    #125970

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC36

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 172_pAAV-ProC37-CatCh-GFP-WPRE

    Plasmid

    #125971

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC37

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 173_pAAV-ProC38-CatCh-GFP-WPRE

    Plasmid

    #125972

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC38

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 174_pAAV-ProC39-CatCh-GFP-WPRE

    Plasmid

    #125973

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC39

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 42_pAAV-ProC41-CatCh-GFP-WPRE

    Plasmid

    #125975

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC41

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 157_pAAV-ProD2-CatCh-GFP-WPRE

    Plasmid

    #125978

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProD2

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EW265 SFFV-mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP (FLP-IN)

    Plasmid

    #236124

    Purpose

    Plasmid encoding fluorescent reporter for PUF-9R binding which expresses mCherry constitutively and EGFP upon stop codon deamination due to ADAR-PUF9R binding

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

    Plasmid

    #183775

    Purpose

    AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    NLS-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid

    #207805

    Purpose

    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

    Depositor

    Koen Breyne

    Article

    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

    Insert

    E-NoMi
    

    Use

    Lentiviral

    Tags

    3xFlag tag, copGFP, mCherry, and nanoluciferase

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

    Plasmid

    #223253

    Purpose

    AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

    Depositor

    Michael Do

    Article

    Liu et al Science. 2023 Jan 27;379(6630):376-381.

    Insert

    macaque OPN4-IRES-EGFP (OPN4 )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin 1 (hSyn)

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

    Plasmid

    #185717

    Purpose

    AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ala 11 to Pro, Val 70 to Pro

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135812

    Purpose

    This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    TagRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Syn-Archon1-KGC-GFP-ER2

    Plasmid

    #115892

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV8

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

    Plasmid

    #87109

    Purpose

    BPNLS-Cas9-BPNLS-2AGFP expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS-2A-EGFP
    

    Use

    CRISPR

    Tags

    3xFLAG (on Cas9)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

    Plasmid

    #111395

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

    Plasmid

    #191690

    Purpose

    For knocking p2a-EGFP into OTX2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    OTX2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

    Plasmid

    #191688

    Purpose

    For knocking p2a-EFGP into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA

    Plasmid

    #191689

    Purpose

    For knocking p2a-EGFP into PTPRO locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    PTPRO homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-EF1a-Archon1-KGC-EGFP-ER2

    Plasmid

    #108425

    Purpose

    Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR-EF1a-Archon2-KGC-EGFP-ER2

    Plasmid

    #108426

    Purpose

    Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR

    Plasmid

    #235204

    Purpose

    Plasmids for constructing mitoBEs v2

    Depositor

    Wensheng Wei

    Article

    Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid

    #84445

    Purpose

    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CAG

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid

    #78174

    Purpose

    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

    Plasmid

    #115890

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

    Plasmid

    #192204

    Purpose

    Lenti-GL(GFP-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-GL(GFP-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65455

    Purpose

    Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid

    #85756

    Purpose

    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Inserts

    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    

    Use

    CRISPR

    Tags

    Csy4, Csy4 recognition sequence, FokI fusion via …

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CMV and U6

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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42. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pTol2-UAS-zArchon1-KGC-EGFP-ER2

    Plasmid

    #108427

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon1-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pTol2-UAS-zArchon2-KGC-EGFP-ER2

    Plasmid

    #108428

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon2-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid

    #190112

    Purpose

    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Inserts

    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    

    Use

    AAV

    Tags

    bpNLS and bpNLS-P2A-eGFP

    Expression

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    EFS and U6, H1

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

    Plasmid

    #175554

    Purpose

    Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    NANOG
    

    Use

    Mouse Targeting

    Tags

    FKBPV-HA2-P2A-eGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

    Plasmid

    #111396

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid

    #108274

    Purpose

    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    EF1α promoter (1.1kb short version)

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. Anti-GFP [N86/38.1R] (Recombinant Antibody)

    Antibody

    #180084-rAb

    Purpose

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Recommended Applications

    Immunocytochemistry, Immunohistochemistry, and Western Blot

    Reactivity

    Synthetic

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available since

    March 29, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

50. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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