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178,426 results

Showing: 141 - 160 of 178426 results

  2. Human sgRNA library Brunello in lentiCRISPRv2

    Pooled library
    #73179
    Purpose
    Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Has service
    Lentiviral Prep
    Expression
    Mammalian
    Use
    CRISPR and Lentiviral
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles

  7. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral prep
    #62723-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato (Cre-dependent)
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    146 Articles
    83480-plasmid-map-sequence-id-423158

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  15. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV1)

    Viral prep
    #100837-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)

    Viral prep
    #52925-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Publication
    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1
    Available since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    72247-plasmid-map-sequence-id-485302

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Showing: 141 - 160 of 178426 results