240,838 results

1. pHR_Gal4UAS_tBFP_PGK_mCherry

   Plasmid

   #79130

   Purpose

   Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   tBFP
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Hylight

   Plasmid

   #193447

   Purpose

   A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

   Depositor

   Richard Goodman

   Article

   Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Hylight Biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVone-AAV9-CMV-eGFP

   Plasmid

   #230928

   Purpose

   pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

   Depositor

   Qizhao Wang

   Article

   Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX_305_Ovalbumin

   Plasmid

   #184924

   Purpose

   Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   Ovalbumin (OVAL Chicken)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Our vector has an A188T mutation that does not im…

   Promoter

   PGK

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAMD1-4

   Plasmid

   #61565

   Purpose

   Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Available Since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAMD1-4

   Plasmid

   #61565

   Purpose

   Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Available Since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTet-O-Ngn2-puro

    Plasmid

    #52047

    Purpose

    3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTet-O-Ngn2-puro

    Plasmid

    #52047

    Purpose

    3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCre

    Plasmid

    #236212

    Purpose

    Arabinose-inducible Cre recombinase

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    Cre recombinase
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Toronto KnockOut version 3 library (TKOv3)

    Pooled Library

    #90294

    Purpose

    Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

    Depositor

    Jason Moffat

    Article

    Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pRK5_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238233

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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