168,838 results

1. pLyGo-Kp-1

   Plasmid

   #163143

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRdSV40-K560-24xGCN4_v4

   Plasmid

   #72229

   Purpose

   A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   K560 kinesin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-UTX-F-MT2

   Plasmid

   #40619

   Purpose

   Expression of enzyme-dead UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTX (KDM6A Human)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   changed Histidine 1146 and Glutamic Acid 1148 to …

   Promoter

   CMV

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET-TRSter

   Plasmid

   #78865

   Purpose

   Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

   Depositor

   Elias Arnér

   Article

   Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

   Insert

   Thioredoxin Reductase (Txnrd1 Rat)
   

   Tags

   Mutated E. coli-type SECIS element (3’ end of ins…

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF3BGX-T2A-lexAp65

   Plasmid

   #138393

   Purpose

   RMCE vector for generating C-terminal T2A-lexA-p65 fusion

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-lexAp65
   

   Expression

   Insect

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

   Plasmid

   #154850

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTA7002-avrPto

   Plasmid

   #49156

   Purpose

   Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

   Depositor

   Sheng Yang He

   Article

   Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Insert

   avrPto
   

   Use

   Plant transformation binary vector

   Promoter

   dexamethasone inducible

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLenti_CMV_PsCatCh2.0-YFP

   Plasmid

   #186978

   Purpose

   Expresses PsCatCh2.0 channelrhodopsin in mammalian cells

   Depositor

   Adam Cohen

   Article

   Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

   Insert

   PsCatCh2.0-YFP
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LTBP1-bio-His

   Plasmid

   #53421

   Purpose

   Expresses full-length Latent-transforming growth factor beta-binding protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LTBP1 (LTBP1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1098 pcDNA GFP FKHR

    Plasmid

    #9022

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR (FOXO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT31

    Plasmid

    #204291

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAT30

    Plasmid

    #204294

    Purpose

    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NES-mCherry-P4Mx1

    Plasmid

    #108143

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLOVE-miR302/367

    Plasmid

    #31310

    Depositor

    Ed Morrisey

    Article

    Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

    Insert

    mmu-miR302/367
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)

    Plasmid

    #199776

    Purpose

    enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    iCre(R297T)
    

    Use

    AAV

    Mutation

    R297T for reduced recombinase efficiency.

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYESir2-puro

    Plasmid

    #1769

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. PTGS1-bio-His

    Plasmid

    #53414

    Purpose

    Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PTGS1 (PTGS1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208038

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSK663

    Plasmid

    #205129

    Purpose

    Expression of GFP-1xPX (from SnxA) in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    1xPX domain of SnxA
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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